|
| Status |
Public on Sep 14, 2012 |
| Title |
OT-2 tcr transgenic + HDAC7 deltaP transgenic, peptide-injected 1 |
| Sample type |
RNA |
| |
|
| Source name |
Flow-sorted CD4/CD8 DP thymocytes, TCR transgenic and HDAC7 mutant transgenic, activated in vivo
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD4/CD8 DP thymocyte
source tissue: thymus
strain: C57 Bl/6
genotype: OT-2 TCR transgenic
treatment: activation by i.p. injection of agonist peptide for OT-2 TCR, 3 hours prior to harvest.
|
| Growth protocol |
Mice were all between 3 and 6 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Qiagen Rneasy Mini kit, with on-column DNAse digestion
|
| Label |
biotin
|
| Label protocol |
Labeling was in accordance with Affymetrix WT labeling kit
|
| |
|
| Hybridization protocol |
In accordance with manufacturer's reccomendation for Gene 1.0ST arrays
|
| Scan protocol |
Affymetrix GCS3000 scanner and the GCOS 1.4 data acquisition software
|
| Data processing |
.cel files were normalized using Expression Console RMA
|
| |
|
| Submission date |
Sep 13, 2012 |
| Last update date |
Sep 14, 2012 |
| Contact name |
Eric Verdin |
| E-mail(s) |
[email protected]
|
| Phone |
(415) 209-2250
|
| Organization name |
Buck Institute for Research on Aging
|
| Department |
NA
|
| Lab |
Verdin
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26488 |
Differential Gene Expression in HDAC7-Deficient and Transgenic Thymocytes |
|
