GEO help: Mouse over screen elements for information.
Status
Public on Nov 21, 2006
Title
small airways, smoker 002, RMA and MAS
Sample type
RNA
Source name
airway epithelial cells obtained by bronchoscopy and brushing
Organism
Homo sapiens Characteristics
age: 46
Extracted molecule
total RNA
Extraction protocol
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
Hybridization protocol
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol
GeneChips were scanned using the GeneChip Scanner 3000 7G .
Description
small airways, smoker 002, RMA and MAS
Data processing
The CEL files were generated using Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings
Submission date
Mar 20, 2006
Last update date
Aug 28, 2018
Contact name
Yael Strulovici-Barel
E-mail(s)
[email protected] Organization name
Weill Cornell Medical College
Department
Department of Genetic Medicine
Lab
Crystal
Street address
1300 York Avenue
City
New York
State/province
NY
ZIP/Postal code
10021
Country
USA
Platform ID
GPL570 Series (9)
GSE4498 Expression data of small airway epithelium from phenotypically normal smokers and non-smokers
GSE5058 Airway epithelium, small airways, normal non-smokers, phenotypic normal smokers, smokers with COPD and early COPD
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD
GSE11784 Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking
GSE17905 Smoking-mediated Up-regulation of GAD67 Expression in the Human Airway Epithelium
GSE18385 Smoking-induced Up-regulation of AKR1B10 Expression in the Airway Epithelium of Healthy Individuals
GSE19407 Smoking-induced Wnt pathway downregulation
GSE20257 Smoking-induced Disarray of the Apical Junctional Complex Gene Expression Architecture in the Human Airway Epithelium
GSE21359 Association of CXCL14 in the Human Airway Epithelium with Chronic Obstructive Lung Disease and Lung Cancer
Relations Reanalyzed by
GSE60486 Reanalyzed by
GSE64985 Reanalyzed by
GSE119087
Data table header descriptions ID_REF Probe set ID
VALUE Signal intensity derived from the CEL files, using RMA method
VALUE_MAS MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE VALUE_MAS ABS_CALL DETECTION P-VALUE 1007_s_at
1862.3304
7036.1123
P
2.19E-04
1053_at
65.823784
363.8456
P
0.009985438
117_at
39.44494
37.95541
A
0.5
121_at
229.41455
637.82367
P
0.003066685
1255_g_at
9.948459
9.234606
A
0.82405084
1294_at
197.59775
960.1741
P
0.011447392
1316_at
59.69895
130.51695
A
0.07383013
1320_at
33.210213
20.329945
A
0.8494729
1405_i_at
155.57756
721.9412
P
9.44E-04
1431_at
13.02307
41.58701
A
0.2674626
1438_at
77.7206
137.77235
P
0.008689182
1487_at
178.04858
871.86365
P
0.035162866
1494_f_at
94.59335
289.0728
P
0.008689182
1552256_a_at
87.0397
317.52255
P
0.0461426
1552257_a_at
176.69855
986.3069
P
7.32E-04
1552258_at
17.230978
93.17653
A
0.27417
1552261_at
78.16771
343.6459
P
0.0375977
1552263_at
69.715515
339.59805
P
2.44E-04
1552264_a_at
173.26123
664.033
P
7.32E-04
1552266_at
17.551489
115.271935
A
0.171387
54675 2210 Kbytes .Supplementary file Size Download File type/resource GSM101107.CEL.gz
8.4 Mb
(ftp) (http) CEL
GSM101107.CHP.gz
13.3 Mb
(ftp) (http) CHP
Processed data included within Sample table Processed data provided as supplementary file
Less...
More...