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| Status |
Public on Nov 21, 2006 |
| Title |
Airway epithelium, small airways, normal non-smokers, phenotypic normal smokers, smokers with COPD and early COPD |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Upregulation of Expression of the Ubiquitin Carboxyl Terminal Hydrolase L1 Gene in Human Airway Epithelium of Cigarette Smokers
The microarray data deposited here is from 39 HG-U133 Plus 2.0 GeneChips, from 12 normal non-smokers, 12 phenotypic normal smokers, 9 Early COPD and 6 COPD individuals, all small airways, all small airway. A subset of these samples have been already submitted under GEO Accession Number GSE 4498. These are: 12 non-smokers samples (GSM101095-GSM101106) and 10 smoker samples (GSM101107-GSM101116). These 22 samples that are also in GSE4498 were described in Harvey, B-G; Heguy, A.; Leopold, P.L.; Carolan, B.; Ferris, B. and Crystal R.G. Modification of Gene Expression of the Small Airway Epithelium in Response to Cigarette Smoking. J. Mol. Med (in press). These data are part of a study aimed at understanding how cigarette smoking modifies neuroendocrine cells, in which microarray analysis with TaqMan confirmation was used to assess airway epithelial samples obtained by fiberoptic bronchoscopy from 81 individuals (normal nonsmokers, normal smokers, smokers with early COPD and smokers with established COPD). Of 11 genes considered to be neuroendocrine cell-specific, only ubiquitin C-terminal hydrolase L1(UCHL1), a member of the ubiquitin proteasome pathway, was consistently upregulated in smokers compared to nonsmokers. Up-regulation of UCHL1 at the protein level was observed with immunohistochemistry of bronchial biopsies of smokers compared to nonsmokers. Interestingly, however, while UCHL1 expression was present only in neuroendocrine cells of the airway epithelium in nonsmokers, UCHL1 expression was also expressed in ciliated epithelial cells in smokers, an intriguing observation in light of recent observations that ciliated cells can are capable of transdifferentiating to other airway epithelium. In the context that UCHL1 is involved in the degradation of unwanted, misfolded or damaged proteins within the cell and is overexpressed in >50% of lung cancers, its overexpression in chronic smokers may represent an early event in the complex transformation from normal epithelium to overt malignancy.
Keywords: non-smokers vs phenotypic normal smokers, smokers with early COPD, and smokers with COPD
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| Overall design |
comparison of gene expression in airway epithelial cells of the small airways of normal non-smokers, phenotypic normal smokers, smokers with early COPD and smokers with COPD
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| Contributor(s) |
Carolan B, Heguy A, Harvey B, Leopold P, Ferris B, Crystal R |
| Citation(s) |
17108109, 19106307 |
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| Submission date |
Jun 12, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
[email protected]
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| Organization name |
Weill Cornell Medical College
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| Department |
Department of Genetic Medicine
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| Lab |
Crystal
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| Street address |
1300 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (39)
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| GSM101095 |
small airways, non-smoker 001, RMA and MAS |
| GSM101096 |
small airways, non-smoker 004, RMA and MAS |
| GSM101097 |
small airways, non-smoker 002, RMA and MAS |
| GSM101098 |
small airways, non-smoker 003, RMA and MAS |
| GSM101099 |
small airways, non-smoker 013, RMA and MAS |
| GSM101100 |
small airways, non-smoker 006, RMA and MAS |
| GSM101101 |
small airways, non-smoker 021, RMA and MAS |
| GSM101102 |
small airways, non-smoker 019, RMA and MAS |
| GSM101103 |
small airways, non-smoker 014, RMA and MAS |
| GSM101104 |
small airways, non-smoker 008, RMA and MAS |
| GSM101105 |
small airways, non-smoker 015, RMA and MAS |
| GSM101106 |
small airways, non-smoker 005, RMA and MAS |
| GSM101107 |
small airways, smoker 002, RMA and MAS |
| GSM101108 |
small airways, smoker 003, RMA and MAS |
| GSM101109 |
small airways, smoker 027, RMA and MAS |
| GSM101110 |
small airways, smoker 033, RMA and MAS |
| GSM101111 |
small airways, smoker 001, RMA and MAS |
| GSM101112 |
small airways, smoker 023, RMA and MAS |
| GSM101113 |
small airways, smoker 048, RMA and MAS |
| GSM101114 |
small airways, smoker 041, RMA and MAS |
| GSM101115 |
small airways, smoker 044, RMA and MAS |
| GSM101116 |
small airways, smoker 049, RMA and MAS |
| GSM114089 |
small airways, smoker 058, MAS5 |
| GSM114090 |
small airways, smoker 063, MAS5 |
| GSM114091 |
small airways, COPD 002, MAS5 |
| GSM114092 |
small airways, COPD 008, MAS5 |
| GSM114093 |
small airways, COPD 012, MAS5 |
| GSM114094 |
small airways, COPD 015, MAS5 |
| GSM114095 |
small airways, COPD 020, MAS5 |
| GSM114096 |
small airways, COPD 021, MAS5 |
| GSM114097 |
small airways, early-COPD 001, MAS5 |
| GSM114098 |
small airways, early-COPD 009, MAS5 |
| GSM114099 |
small airways, early-COPD 012, MAS5 |
| GSM114100 |
small airways, early-COPD 020, MAS5 |
| GSM114101 |
small airways, early-COPD 037, MAS5 |
| GSM114102 |
small airways, early-COPD 045, MAS5 |
| GSM114103 |
small airways, early-COPD 054, MAS5 |
| GSM114104 |
small airways, early-COPD 064, MAS5 |
| GSM114105 |
small airways, early-COPD 086, MAS5 |
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| This SubSeries is part of SuperSeries: |
| GSE5060 |
Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD |
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| Relations |
| BioProject |
PRJNA104499 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5058_RAW.tar |
809.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
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