|
| Status |
Public on Oct 11, 2012 |
| Title |
Col-0 vs. triple mutant, replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
wild type
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
tissue: vegetative part
age: stage 1.04
genotype/variation: WT
|
| Growth protocol |
Plants were grown on MS medium under a 16 h light/ 8 h dark cycle at 22°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Agilent's Low RNA Input Linear Amplification kit PLUS.
|
| |
|
| Channel 2 |
| Source name |
shr scl23 scr
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
tissue: vegetative part
age: stage 1.04
genotype/variation: shr-2 scl23-1 scr-5
|
| Growth protocol |
Plants were grown on MS medium under a 16 h light/ 8 h dark cycle at 22°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's protocol.
|
| Label |
Cy5
|
| Label protocol |
Agilent's Low RNA Input Linear Amplification kit PLUS.
|
| |
|
| |
| Hybridization protocol |
Standard Agilent's Gene Expression Hybridization Kit protocol.
|
| Scan protocol |
Images were scanned using Agilent's DNA microarray scanner and quantified using Feature Extraction Software.
|
| Description |
Col-0 vs triple 3 vs 4
|
| Data processing |
Agilent's GeneSpring Software was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Oct 10, 2012 |
| Last update date |
Oct 11, 2012 |
| Contact name |
Eun Kyung Yoon |
| E-mail(s) |
[email protected]
|
| Organization name |
KonKuk University
|
| Street address |
120 Neungdong-ro Gwangjin-gu
|
| City |
Seoul |
| ZIP/Postal code |
143-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE41478 |
Arabidopsis thaliana vegetative part: Col-0 vs. shr scl23 scr triple mutant |
|
