|
| Status |
Public on Oct 17, 2012 |
| Title |
PMN-MDSC RMA-S blood, repl. 3 |
| Sample type |
RNA |
| |
|
| Source name |
RMA-S mouse blood
|
| Organism |
Mus musculus |
| Characteristics |
cell type: polymorphnuclear-MDSC from blood
treatment: RMA-S cell line
genetic background: C57BL/6
age: 9 wk
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy Mini Kit (Qiagen), followed by DNase I treatment (TURBO DNA-free kit, Ambion). Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation
|
| |
|
| Submission date |
Oct 16, 2012 |
| Last update date |
Oct 17, 2012 |
| Contact name |
Christian Eisen |
| E-mail(s) |
[email protected]
|
| Organization name |
German Cancer Research Center
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE41620 |
Tumor-infiltrating monocytic myeloid-derived suppressor cells mediate CCR5-dependent recruitment of regulatory T cells favoring tumor growth |
|
