|
| Status |
Public on Nov 22, 2012 |
| Title |
Ex vivo spleen CD4-CD8- DCs rep1 |
| Sample type |
RNA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
gender: female
age: 6 weeks
pool of x animals: 8
replicate: biological replicate 1
surface markers: CD11c+, CD3-, CD4-, CD8-
|
| Treatment protocol |
Cells were isolated ex vivo and cells were directly isolated within a total range of time of 5h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The miRNeasy total RNA extraction kit was used for RNA isolation. DNase treatment was performed on columns according to the instructions of the manufacturer.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled miRNA was prepared from 100ng total RNA using Agilent miRNA Complete Labeling and Hyb Kit according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized with a 8X15 mouse miRNA array rel 12 v2 (design number 021828) for chip 1 and 8X15 mouse miRNA array rel v15 (design number 029152) for chip 2 at 55°C and 20 rpm for 20 hours, and then washed according to the manufacturer’s instructions.
|
| Scan protocol |
Agilent Technologies Scanner G2505B US22502692 using 5uM resolution
|
| Description |
miRNA expression profile of mouse CD4-CD8- DCs
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol miRNA_107_Sep09 and Grid: 029152_D_F_20100702) to obtain background subtracted and spatially detrended Processed Signal intensities.
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Nov 22, 2012 |
| Contact name |
Alexander Mildner |
| E-mail(s) |
[email protected]
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Immunology
|
| Street address |
234 Herzl street
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL10384 |
| Series (1) |
| GSE42434 |
microRNA expression profiling of various C57Bl/6 myeloid cell populations |
|
