|
| Status |
Public on Dec 03, 2013 |
| Title |
201B6(p25)_undiff_hiPS |
| Sample type |
genomic |
| |
|
| Source name |
undifferentiated hiPS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: undiff_hiPS
method: Retrovirus
cell origin: Fibro (HDF1388)
transduction: OSKM
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extraction and purification from cultured cells was carried out using a Gentra Puregene kit (QIAGEN).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting.
|
| Data processing |
Illumina GenomeStudio software
Unmethylated(Signal_A) and methylated(Signal_B) signal intensities
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Dec 03, 2013 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
[email protected]
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8490 |
| Series (2) |
| GSE42448 |
Comparison of DNA methylation profilings between hESCs and hiPSCs |
| GSE49053 |
Differentiation defective phenotypes revealed by large scale analyses of human pluripotent stem cells |
|
