|
Status |
Public on Dec 03, 2013 |
Title |
404C2(p48)_undiff_hiPS |
Sample type |
genomic |
|
|
Source name |
undifferentiated hiPS
|
Organism |
Homo sapiens |
Characteristics |
cell type: undiff_hiPS method: Episomal plasmid cell origin: Fibro (HDF1388) transduction: OSKUL+shp53
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA extraction and purification from cultured cells was carried out using a Gentra Puregene kit (QIAGEN).
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol.
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting.
|
Data processing |
Illumina GenomeStudio software Unmethylated(Signal_A) and methylated(Signal_B) signal intensities
|
|
|
Submission date |
Nov 21, 2012 |
Last update date |
Dec 03, 2013 |
Contact name |
Shinya Yamanaka |
E-mail(s) |
[email protected]
|
Phone |
81-75-366-7041
|
Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
City |
Kyoto |
ZIP/Postal code |
606-8507 |
Country |
Japan |
|
|
Platform ID |
GPL8490 |
Series (2) |
GSE42448 |
Comparison of DNA methylation profilings between hESCs and hiPSCs |
GSE49053 |
Differentiation defective phenotypes revealed by large scale analyses of human pluripotent stem cells |
|