|
| Status |
Public on Dec 03, 2013 |
| Title |
TKCBV5-6_undiff.hiPS_SFEBq_defective |
| Sample type |
RNA |
| |
|
| Source name |
undifferentiated hiPS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: undiff_hiPS
method: Retrovirus
cell origin: CB CD34-/CD45+
transduction: OSKM
sfebq: differentiation defective
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Approximately 5.5ug of labeled DNA target was hybridized to the Affymetrix GeneChip Human Exon 1.0 ST Array at 45℃ for 17 hrs according to the manufacturer's recommendation.
|
| Scan protocol |
Hybridized arrays were washed and satined on a GeneChip Fluidics Satation 450 and scanned on a GCS3000 Scanner (Affymetrix).
|
| Data processing |
The exon array data analysis was performed using the Gene Spring GX11.5.1 software. Summarization algorithme:ExonPLIER16 with core and extended probe sets.
probe group file: HuEx-1_0-st-v2_na32_hg19_2011-06-23
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Dec 03, 2013 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
[email protected]
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL5175 |
| Series (2) |
| GSE42449 |
Exon array analysis for SFEBq differentiation-defective clones and good clones |
| GSE49053 |
Differentiation defective phenotypes revealed by large scale analyses of human pluripotent stem cells |
|
