|
| Status |
Public on Dec 03, 2013 |
| Title |
414C2(p54)_undiff.hiPS_SFEBq_good |
| Sample type |
RNA |
| |
|
| Source name |
undifferentiated hiPS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: undiff_hiPS
method: Episomal plasmid
cell origin: Fibro (HDF1388)
transduction: OSKUL+shp53
sfebq: differentiation good
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
|
| Label |
Cy3
|
| Label protocol |
Agilent standard protocol
|
| |
|
| Hybridization protocol |
Agilent standard protocol
|
| Scan protocol |
Agilent standard protocol
|
| Data processing |
The data were analyzed using the Gene Spring GX 11.5.1 software program (Agilent Technologies).The data processing was performed as follows; (i) Threshold raw signals were set to 1.0, (ii) Log base 2 transformation was performed, (iii) 75th percentile normalization was chosen as the normalized algorithm.
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Dec 03, 2013 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
[email protected]
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE42452 |
Comparison of gene expression profilings between SFEBq differentiation-defective clones and good clones |
| GSE49053 |
Differentiation defective phenotypes revealed by large scale analyses of human pluripotent stem cells |
|
