|
| Status |
Public on Jun 01, 2013 |
| Title |
SVR, sham, 0h (rep 3) |
| Sample type |
RNA |
| |
|
| Source name |
sham, 0h
|
| Organism |
Mus musculus |
| Characteristics |
cell line: SVR
cell type: angiosarcoma
|
| Treatment protocol |
Cells were treated with sham (t=0 hrs) or 50 micromolar propranolol (t=4 & 24 hrs)
|
| Growth protocol |
SVR cells were cultured to 100% confluence in T75 flasks in Dulbecco's modified Eagle's media (DMEM) supplemented with 10% fetal bovine serum (FBS), 80 U/ml penicillin, and 50 micrograms/ml streptomycin C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using the Pulink RNA mini kit (Ambion) according to manufacturer's instructions. Quality control was performed with the Nanodrop and Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified and biotin-labeled using Illumina TotalPrep RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
750 ng of biotinylated aRNA was then briefly heat-denatured and loaded onto Illumina expression arrays to hybridize overnight.
|
| Scan protocol |
Illumina ISCAN.
|
| Description |
8090936018_C
|
| Data processing |
Intensity values were transferred to Agilent GeneSpring GX microarray analysis software and data was filtered based on quality of each call.
|
| |
|
| Submission date |
Nov 27, 2012 |
| Last update date |
Jun 01, 2013 |
| Contact name |
Brad Allen Bryan |
| E-mail(s) |
[email protected]
|
| Organization name |
Texas Tech University Health Sciences Center
|
| Department |
Biomedical Sciences
|
| Street address |
5001 El Paso Drive
|
| City |
El Paso |
| State/province |
Texas |
| ZIP/Postal code |
79905 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE42534 |
Genome-wide analysis of the effects of propranolol on angiosarcoma tumor cells |
|
