|
| Status |
Public on Feb 05, 2013 |
| Title |
COLON APCmin/+/VP16 (CTRL) 4 |
| Sample type |
RNA |
| |
|
| Source name |
Intestinal (colon) Tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J-FVBN Mixed Strain
age: 8 months
tissue: Intestinal (colon) Tumor
genotype/variation: APCmin/+/VP16 (control)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAzol reagent, following manufacturer's instructions. Quality control was performed with Biorad Experion.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina® TotalPrepTM RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
COLON APCmin/+/VP16 (CTRL) 4
replicate 1
|
| Data processing |
The data were normalised using quantile normalization with Illumina Genome Studio Software; items with detection p values >0.001 or AVG Signal <100 in both groups were excluded.
|
| |
|
| Submission date |
Feb 05, 2013 |
| Last update date |
Feb 05, 2013 |
| Contact name |
Antonio Moschetta |
| Organization name |
University of Bari & Consorzio Mario Negri Sud
|
| Street address |
Via Nazionale 8A
|
| City |
Santa Maria Imbaro |
| ZIP/Postal code |
66030 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE44070 |
Genome-wide analysis of gene expression profile of Intestinal (COLON) Tumors from APCmin/+/VP16LXRa vs APCmin/+/VP16 |
| GSE44073 |
Liver X Receptors play an antitumoral role in the intestine |
|
