|
| Status |
Public on Mar 26, 2013 |
| Title |
N. gonorrhoeae grown in KSFM Rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
N. gonorrhoeae grown in KSFM
|
| Organism |
Neisseria gonorrhoeae F62 |
| Characteristics |
strain: F62
specific growth conditions: Grown for 120 mInutes in KSFM, static with 5% CO2
|
| Growth protocol |
Grown for 120 mInutes in KSFM, static with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TRIzol (phenol/chloroform) according to instructions
Libraries were constructed with BioChain's mRNA Sample Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
Rockhopper version 1.20 (manuscript submitted) was used for alignment, normalization, and quantification. Rockhopper is available for download from http://cs.wellesley.edu/~btjaden/Rockhopper
Genome_build: FA 1090 genome (NC_002946.2)/ASM16353v1
Supplementary_files_format_and_content: Tab delimited text files containing transcription coordinates and abundance estimates.
|
| |
|
| Submission date |
Mar 23, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian Tjaden |
| Organization name |
Wellesley College
|
| Street address |
106 Central Street
|
| City |
Wellesley |
| State/province |
MA |
| ZIP/Postal code |
02481 |
| Country |
USA |
| |
|
| Platform ID |
GPL16863 |
| Series (1) |
| GSE45444 |
Computational Analysis of Bacterial RNA-Seq Data |
|
| Relations |
| SRA |
SRX254788 |
| BioSample |
SAMN01990865 |
