|
| Status |
Public on Apr 07, 2014 |
| Title |
ddm1 F6 inflorescence replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
ddm1, F6 inbred generation, inflorescence, unopened flower buds
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: unopened flower buds
genotype/variation: ddm1, F6 inbred generation
|
| Growth protocol |
Plants were grown on soil in long day (18 hour) light at 23C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from unopened flower buds using Trizol reagent. Total RNA was further purified using a Qiagen MinElute RNA cleanup kit.
|
| Label |
biotin
|
| Label protocol |
Total RNA were assessed for quality with Agilent 2100 Bioanalyzer and Nanodrop 8000 spectrophotometer. Hybridization targets were prepared with MessageAmp Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was performed to ATH1 arrays in Affymetrix GeneChip® hybridization oven 645 and washed in Affymetrix GeneChip® Fluidics Station 450 according to standard Affymetrix GeneChip® Hybridization and Wash protocols.
|
| Scan protocol |
Arrays were scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix protocols.
|
| Description |
ddm1 F6
|
| Data processing |
The data were analyzed with the GeneSpring GX Software Suite using GC-RMA normalization
|
| |
|
| Submission date |
Apr 15, 2013 |
| Last update date |
Apr 07, 2014 |
| Contact name |
Richard Keith Slotkin |
| E-mail(s) |
[email protected]
|
| Organization name |
The Ohio State University
|
| Department |
Molecular Genetics
|
| Lab |
Slotkin Laboratory
|
| Street address |
318 West 12th Ave.
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE46050 |
Gene expression in Arabidopsis ddm1 mutants with high levels of Transposable Element activity |
|
