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Sample GSM114095 Query DataSets for GSM114095
Status Public on Nov 21, 2006
Title small airways, COPD 020, MAS5
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 57
Sex: M
Ethnic group: white
Smoking Status: COPD, GOLD-I, 20 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G
Description small airways, COPD 020, MAS5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 12, 2006
Last update date Aug 28, 2018
Contact name Yael Strulovici-Barel
E-mail(s) [email protected]
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (4)
GSE5058 Airway epithelium, small airways, normal non-smokers, phenotypic normal smokers, smokers with COPD and early COPD
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD
GSE8545 Variability in Small Airway Epithelial Gene Expression Among Normal Smokers
Relations
Reanalyzed by GSE60486
Reanalyzed by GSE119087

Data table header descriptions
ID_REF Probe set ID
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 8950.5 P 0.000219
1053_at 339.7 P 0.024711
117_at 127.8 A 0.175989
121_at 486.7 P 0.013092
1255_g_at 17.1 A 0.541184
1294_at 842.7 P 0.000468
1316_at 238.5 P 0.002228
1320_at 17.8 A 0.933135
1405_i_at 1016.8 P 0.000944
1431_at 11.2 A 0.849473
1438_at 169.7 A 0.098054
1487_at 1034.2 P 0.035163
1494_f_at 483.6 P 0.011447
1552256_a_at 370.3 P 0.023926
1552257_a_at 919.7 P 0.001953
1552258_at 16.6 A 0.72583
1552261_at 224.5 A 0.149658
1552263_at 539.3 P 0.001221
1552264_a_at 517.2 P 0.001221
1552266_at 134.1 A 0.219482

Total number of rows: 54675

Table truncated, full table size 1445 Kbytes.




Supplementary file Size Download File type/resource
GSM114095.CEL.gz 8.1 Mb (ftp)(http) CEL
GSM114095.CHP.gz 13.1 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
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