|
| Status |
Public on May 22, 2013 |
| Title |
Strain PHC, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
photoheterotrophically grown R.sphaeroides cells
|
| Organism |
Cereibacter sphaeroides |
| Characteristics |
strain: strain PHC
|
| Growth protocol |
R.sphaeroides cells were grown photoheterotrophically at 30 oC in Ormerod's medium containing malate as a carbon source, under incadescent illumination.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Midi Kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol with ddNTP labeling kit
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
gene expression data from strain PHC
|
| Data processing |
generation of CEL files with Affymetrix MAS5.0, expression computation using RMA
|
| |
|
| Submission date |
May 21, 2013 |
| Last update date |
May 22, 2013 |
| Contact name |
Oleg Moskvin |
| Organization name |
University of Wisconsin-Madison
|
| Street address |
1220 Capitol Court
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53715 |
| Country |
USA |
| |
|
| Platform ID |
GPL162 |
| Series (1) |
| GSE47149 |
Mutations in GlnA, NifA and NifK enabling photosynthetic growth of Rhodobacter sphaeroides in the absence of CO2 fixation |
|
