|
| Status |
Public on Jun 24, 2006 |
| Title |
hTERT_EXTENDED_HD1_REP1 |
| Sample type |
RNA |
| |
|
| Source name |
CD8 T lymphocytes
|
| Organism |
Homo sapiens |
| Characteristics |
late passage CD8 T cells clones expressing hTERT.
|
| Treatment protocol |
Mycoplasma-free cells were harvested after 15 to 18 days of mitogen stimulation (PHA), Annexin-V expression <10%.
|
| Growth protocol |
Cell are cultures in RPMI 1640 medium supplemented with 8% Human Serum and 150U/ml of rIL-2 (GlaxoSmithKline) and stimulated using PHA (Sodiag) and irradiated feeder cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from 30x10E6 cells was extracted, purified and used for cDNA synthesis following standard procedures (www.Affymetrix.com).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 micrograms total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr on human U133A GeneChip, Chips were washed and stained in the Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
|
| Description |
hTERT expressing transduced CD8 T Lymphocytes, late passage, healthy donor 1, rep1
|
| Data processing |
The .cel files data were analyzed with the R library affy, using RMA default settings and the quantile normalization method.
|
| |
|
| Submission date |
Jun 23, 2006 |
| Last update date |
Jun 23, 2006 |
| Contact name |
Mauro Delorenzi |
| E-mail(s) |
[email protected]
|
| Organization name |
Swiss Institute of Bioinformatics
|
| Lab |
BCF
|
| Street address |
Génopode Building, Quartier Sorge
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE5142 |
hTERT effects in CD8 T Lymphocytes HD1 |
| GSE5144 |
hTERT effects in CD8 T Lymphocytes from two healthy donors |
|
