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| Status |
Public on Jun 24, 2006 |
| Title |
hTERT effects in CD8 T Lymphocytes from two healthy donors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Expression in GFP vs. GFP/hTERT transduced CD8 T Lymphocytes from Healty Donors (HD) 1 and 2 at early and late passages. Using CD8+ T lymphocyte clones over-expressing telomerase we investigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone.
Keywords: Cell Line Comparison.
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| Overall design |
triplicates of 4 conditions for each of 2 donors, 1 chip (hTERT_YOUNG_HD1_REP2) of the first donor excluded because of low quality hybridization, one condition (GFP YOUNG)lost from second donor because of problems in cell culture.
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| |
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| Contributor(s) |
Rufer N, Menzel O, Migliaccio M, Goldstein D, Dahoun S, Delorenzi M |
| Citation(s) |
16951325 |
| |
| Submission date |
Jun 23, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Mauro Delorenzi |
| E-mail(s) |
[email protected]
|
| Organization name |
Swiss Institute of Bioinformatics
|
| Lab |
BCF
|
| Street address |
Génopode Building, Quartier Sorge
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA96659 |
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