|
| Status |
Public on Jun 01, 2016 |
| Title |
SporadicPRCC2_NormalTissue_5 |
| Sample type |
RNA |
| |
|
| Source name |
Normal tissue from sporadic PRCC2 patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: Normal
disease status: sporadic PRCC2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions, followed by purification using the Qiagen RNAeasy kit.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol, 10ug of RNAeasy-purified total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
|
| Description |
Gene expression profiling of hereditary and sporadic Type 2 Papillary Renal Cell Carcinoma (PRCC2) and adjacent normal tissue from PRCC2 patients
|
| Data processing |
CEL files were loaded into R and then preprocessed and normalized using RMA. A custom CDF (GPL16311) was used to reannotate the probes.
|
| |
|
| Submission date |
Jun 27, 2013 |
| Last update date |
Jun 01, 2016 |
| Contact name |
Weng Khong Lim |
| E-mail(s) |
[email protected]
|
| Organization name |
National Cancer Centre Singapore
|
| Department |
Department of Medical Sciences
|
| Lab |
NCCS-VARI Translational Research Laboratory
|
| Street address |
11 Hospital Drive
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL16311 |
| Series (1) |
| GSE48352 |
DPYS as a potential diagnostic biomarker in hereditary and sporadic PRCC2 |
|
