|
| Status |
Public on Mar 26, 2014 |
| Title |
KO TH2 cells |
| Sample type |
RNA |
| |
|
| Source name |
KO TH2 cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Bhlhe40 KO
cell type: purified splenic CD4 T cells
cell culture conditions: TH2 conditions for 4 days
treatment: stimulated with PMA and ionomycin for 4 hours prior to collection of total RNA
|
| Treatment protocol |
Cells were stimulated with PMA and ionomycin for 4 hours prior to collection of total RNA.
|
| Growth protocol |
Purified naïve CD4+B220-CD25-CD44loCD62Lhi cells were cultured in TH0, TH1, TH2, or TH17 conditions for 4 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by the E.Z.N.A. MicroElute Total RNA Kit, Omega Bio-Tek.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified (Ovation Pico SL WTA System V2, Nugen), labeled (Encore Biotin Module, Nugen), and fragmented.
|
| |
|
| Hybridization protocol |
Hybridization was performed by standard methods at the GTAC at Washington University School of Medicine.
|
| Scan protocol |
GeneChips were scanned at the GTAC at Washington University School of Medicine.
|
| Description |
Gene expression data from KO TH2 cells
|
| Data processing |
Array data were imported into Arraystar 5 software (DNAstar) and then normalized using the robust multi-array analysis method with quantile normalization.
|
| |
|
| Submission date |
Jul 24, 2013 |
| Last update date |
Mar 27, 2014 |
| Contact name |
Chih-Chung Lin |
| E-mail(s) |
[email protected]
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Lab |
Edelson Lab
|
| Street address |
660 S. Euclid Ave. Box 8118
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE49166 |
Expression data from mouse differentiated T helper cell lineages |
|
