|
| Status |
Public on Aug 13, 2013 |
| Title |
WT 1 Lin- |
| Sample type |
RNA |
| |
|
| Source name |
Wild type lineage negative bone marrow #1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
|
| Treatment protocol |
Bone marrow was extracted from mouse femur, washed with PBS and then either taken directly for RNA extraction or first lineage depleted using a lineage depletion kit (Miltenyi Biotec).
|
| Growth protocol |
ERG transgenic animals were kept in the animal facility and were sacrified when leukemia signs appeared.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TriZol, according to the manufacturer's instructions and cleaned with Phenol/chlorophorm/Isoamyl alcohol in Phase lock gel (PLG) tubes.
|
| Label |
biotin
|
| Label protocol |
Affymetrix biotin labeling_ wt kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Affymetrix wt labeling protocol as described in https://www.affymetrix.com/support/downloads/manuals/wt_sensetarget_label_manual.pdf
|
| Scan protocol |
Affymetrix wt scanning protocol as described in https://www.affymetrix.com/support/downloads/manuals/wt_sensetarget_label_manual.pdf
|
| Data processing |
RMA_PARTEK
|
| |
|
| Submission date |
Aug 12, 2013 |
| Last update date |
Oct 25, 2022 |
| Contact name |
jasmine Jacob |
| E-mail(s) |
[email protected]
|
| Phone |
0523790500
|
| Organization name |
Sheba Medical Center at Tel HaShomer
|
| Street address |
haChevel 33
|
| City |
Shoham |
| State/province |
-Select- |
| ZIP/Postal code |
60850 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE49787 |
Expression data of leukemia samples taken from transgenic ERG mice |
|
