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Sample GSM120830 Query DataSets for GSM120830
Status Public on Jul 22, 2006
Title Gaido_Epididymus_gd16_9_25May2006
Sample type RNA
 
Source name Epididymus
Organism Mus musculus
Characteristics gene reported: wild-type
strain: CD-1
Sex: Male
developmental stage: gd 16
theiler stage: 24
somite count: NA
developmental landmark: NA
Treatment protocol pool size: NA
Pooled sample: No
Dissection Method: Whole organ excision
Experimental Design: CD-1 mice are time-mated within a 2 hour window. Pregnant CD-1 mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal epididymides are removed from fetuses at gestation days 12, 14, 16, 18, and postnatal day 2, snap frozen in liquid nitrogen and stored at -80ÂșC.
Extracted molecule total RNA
Extraction protocol Gaido protocol: 'RNA isolation from whole testes'
Label Biotin
Label protocol -
 
Hybridization protocol Affymetrix standard protocol (701021 Rev 4)
Amount labeled target hybridization to array: 15 ug
Scan protocol -
Description >> Amplification protocol <<
Target Amplified manufacturer/kit: Affymetrix
Target Amplified protocol: Affymetrix Two-Cycle cDNA synthesis protocol (701025 Rev 5)
Rounds of amplification: 2
URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:2209
Data processing Analysis method: Affymetrix GCOS and SAS Microarray Solutions GCOS Tgt value: 500
 
Submission date Jul 18, 2006
Last update date Aug 28, 2018
Contact name GUDMAP Developers
E-mail(s) [email protected]
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE5333 Gene expression profiling of whole epididymis in embryonic (gestational day 12, 14, 16 & 18) and postnatal day 2 mice. (GUDMAP Series ID: 4)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 572.16 P 0.000754
AFFX-BioB-M_at 736.91 P 0.000754
AFFX-BioB-3_at 546.94 P 0.000044
AFFX-BioC-5_at 1653.78 P 0.000390
AFFX-BioC-3_at 1606.03 P 0.000044
AFFX-BioDn-5_at 3205.83 P 0.000044
AFFX-BioDn-3_at 5961.23 P 0.000052
AFFX-CreX-5_at 16929.34 P 0.000052
AFFX-CreX-3_at 19753.87 P 0.000044
AFFX-DapX-5_at 176.82 P 0.000390
AFFX-DapX-M_at 1553.59 P 0.000754
AFFX-DapX-3_at 2719.54 P 0.000052
AFFX-LysX-5_at 8.12 A 0.500000
AFFX-LysX-M_at 214.59 P 0.002023
AFFX-LysX-3_at 575.30 P 0.000052
AFFX-PheX-5_at 112.23 P 0.004998
AFFX-PheX-M_at 336.84 P 0.000662
AFFX-PheX-3_at 649.23 P 0.000070
AFFX-ThrX-5_at 70.32 A 0.116113
AFFX-ThrX-M_at 277.48 P 0.000110

Total number of rows: 45101

Table truncated, full table size 1278 Kbytes.




Supplementary file Size Download File type/resource
GSM120830.CEL.gz 6.0 Mb (ftp)(http) CEL

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