|
| Status |
Public on Apr 10, 2014 |
| Title |
TIG3-Ras/TIG3-Vector |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
TIG3-Vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human lung embryonic fibroblast
cell line: TIG-3
transduction: empty vector
|
| Treatment protocol |
TIG-3 cells were subjected to infection with retrovirus encoding RasV12 or empty vector.
|
| Growth protocol |
TIG-3 cells were cultured in DME supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol according to the manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Channel 2 |
| Source name |
TIG3-Ras
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human lung embryonic fibroblast
cell line: TIG-3
transduction: RasV12
|
| Treatment protocol |
TIG-3 cells were subjected to infection with retrovirus encoding RasV12 or empty vector.
|
| Growth protocol |
TIG-3 cells were cultured in DME supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| |
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner (Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Data processing |
F635 Median column as Red signal and F532 Median column as Green signal. Global normalized, background subtracted data obtained from log2 of processed Green signal/processed Red signal.
|
| |
|
| Submission date |
Aug 13, 2013 |
| Last update date |
Apr 10, 2014 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE49862 |
Gene expression profile of oncogenic Ras-induced senescence in normal human diploid fibroblasts. |
|
