|
| Status |
Public on Dec 06, 2013 |
| Title |
Lactation Adipose Stroma, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Lactation day 5
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
development stage: Lactation
tissue: mammary gland stroma
|
| Treatment protocol |
Adipose stroma was isolated from the mouse mammary gland via a collagenase hyaluronidase digestion and subsequent centrifugation.
|
| Growth protocol |
Female Balb/c mice were bred to generate lactating or involuting mice. Nulliparous mice were not exposed to male mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
|
| |
|
| Hybridization protocol |
Samples were hybridized for 16 hr at 45C. The array was then washed and stained using the fluidics station.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 with Autoloader
|
| Data processing |
Normalization and differential expression were carried out with Affy, gcrma and Limma packages in the Bioconductor suite of programs
|
| |
|
| Submission date |
Dec 05, 2013 |
| Last update date |
Dec 06, 2013 |
| Contact name |
Jessica McCready |
| E-mail(s) |
[email protected]
|
| Organization name |
Assumption College
|
| Street address |
500 Salisbury St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01609 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE53044 |
Expression Data from Mouse Mammary Gland Adipose Stroma |
|
