|
| Status |
Public on Dec 11, 2013 |
| Title |
Whole fly tissue: hatched and raised in earth gravity bacterial infection biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Whole homogenized drosophila infected with Escherichia coli
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Oregon R
tissue: whole organism
life stage: adult
infection: infected with Escherichia coli
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Qiagen Rneasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard protocols described in the Affymetrix Gene Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila mlanogaster 3' IVT arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from a pool of 4-20 whole flies hatched and raised in earth gravity, infected with E. coli
|
| Data processing |
The data were analyzed with Partek Genomics Suite
|
| |
|
| Submission date |
Dec 10, 2013 |
| Last update date |
Dec 11, 2013 |
| Contact name |
Michael George |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California, Davis
|
| Department |
Medical Microbiology and Immunology
|
| Street address |
451 Health Sciences Drive
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE53196 |
Expression data from drosophila melanogaster |
|
