|
| Status |
Public on Feb 18, 2014 |
| Title |
Islet replicate 2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Dam-Islet processed DNA from stage 17 embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: w-; + ; UAS-Dam-Islet
tissue: stage 17 embryos
|
| Treatment protocol |
Embryos were collected, washed and frozen on dry ice.
|
| Growth protocol |
Embryos were collected for 6 hours and allowed to develop for a further 16 hours (at 25oC).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tissues were dissected and placed n 180ul of PBS with 100mM EDTA before samples were processed as previously described (Choksi et al., 2006)
|
| Label |
Cy3
|
| Label protocol |
Labeling has been done using the Nimbelgen Dual-Color Labeling Kit based on a Klenow enzymatic reaction following manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
Dam processed DNA from stage 17 embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: w-; + ; UAS-Dam
tissue: stage 17 embryos
|
| Treatment protocol |
Embryos were collected, washed and frozen on dry ice.
|
| Growth protocol |
Embryos were collected for 6 hours and allowed to develop for a further 16 hours (at 25oC).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tissues were dissected and placed n 180ul of PBS with 100mM EDTA before samples were processed as previously described (Choksi et al., 2006)
|
| Label |
Cy5
|
| Label protocol |
Labeling has been done using the Nimbelgen Dual-Color Labeling Kit based on a Klenow enzymatic reaction following manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
Hybridisation has been done with HX1 mixer following Nimblegen's procedures
|
| Scan protocol |
Arrays were scanned by Nimblegen
|
| Description |
Dam-Islet / Dam-control in stage 17 embryos
|
| Data processing |
The Dam-fusion/Dam log2 ratios were median normalised
|
| |
|
| Submission date |
Dec 18, 2013 |
| Last update date |
Feb 18, 2014 |
| Contact name |
Tony David Southall |
| E-mail(s) |
[email protected]
|
| Organization name |
Imperial College London
|
| Department |
Life Sciences
|
| Lab |
Southall lab
|
| Street address |
South Kensington Campus
|
| City |
London |
| ZIP/Postal code |
SW7 2AZ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10639 |
| Series (1) |
| GSE53446 |
Profiling of Islet, Lim3, Hb9 and Eve genomic binding in Drosophila with DamID |
|
