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Sample GSM130728 Query DataSets for GSM130728
Status Public on Sep 13, 2006
Title D. simulans pure-species adults, D. simulans pure-species adults, genome chip, replicate 1, Cy5
Sample type RNA
 
Source name Drosophila simulans males, 4 days old
Organism Drosophila simulans
Characteristics Drosophila simulans
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol RNA isolation was accomplished with Qiagen RNeasy Mini Kit reagents. The RNA (10 µg) was annealed at the Duke Microarray Core Facility.
Label Cy5
Label protocol The labelling was done at the Duke Microarray Core Facility.
 
Scan protocol Fluorescent array images were collected at the Duke Microarray Core Facility.
Description D. simulans pure-species adults, D. simulans pure-species adults, genome chip
Data processing The median pixel feature intensity after background subtraction (EXPRESSION column) was log2 transformed, and normalized on the median for that hybridization (giving the VALUE column) to obtain the values given in the data table.
 
Submission date Aug 24, 2006
Last update date Jun 12, 2013
Contact name Amanda J. Moehring
E-mail(s) [email protected]
Organization name Duke University
Department DCMB/Biology
Lab Mohamed Noor
Street address Box 91000
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL3829
Series (3)
GSE5601 Comparison of D. simulans/D. mauritiana F1 male hybrid genome-wide expression values to pure-species parents
GSE5607 Comparison of D. simulans/D. sechellia F1 male hybrid genome-wide expression values to pure-species parents
GSE5655 Comparison of pure-species Drosophila expression to hybrid expression

Data table header descriptions
ID_REF clone number for genes with expression in Channel 1
EXPRESSION The channel 1 median pixel feature intensity after background subtraction
VALUE The channel 1 EXPRESSION column after being log2 transformed and normalized on the median for that hybridization.

Data table
ID_REF EXPRESSION VALUE
1773 221 1.129691076
4472 133 0.397070953
14407 1884 4.221371767
604 372 1.880947328
13564 498 2.301790449
3422 106 0.069708972
2669 37 -1.448758117
9292 100 -0.014355293
5201 475 2.23357222
11062 88 -0.198779864
1560 143 0.501659854
5089 91 -0.150416843
6766 197 0.963840336
1131 178 0.817521948
4379 62 -0.704015173
11156 82 -0.300659478
9798 172 0.768053272
4531 127 0.330473204
8880 3212 4.991044697
11455 77 -0.391424942

Total number of rows: 15552

Table truncated, full table size 319 Kbytes.




Supplementary data files not provided

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