|
| Status |
Public on Sep 13, 2006 |
| Title |
D. simulans/D. sechellia hybrids, D. simulans/D. sechellia hybrid adults, genome chip, replicate 1, Cy5 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila simulans/D. sechellia F1 hybrid males, 4 days old
|
| Organism |
Drosophila sechellia x Drosophila simulans |
| Characteristics |
Drosophila simulans/D. sechellia F1 hybrids
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was accomplished with Qiagen RNeasy Mini Kit reagents. The RNA (10 µg) was annealed at the Duke Microarray Core Facility.
|
| Label |
Cy5
|
| Label protocol |
The labelling was done at the Duke Microarray Core Facility.
|
| |
|
| Scan protocol |
Fluorescent array images were collected at the Duke Microarray Core Facility.
|
| Description |
D. simulans/D. sechellia hybrids, D. simulans/D. sechellia hybrid adults, genome chip
|
| Data processing |
The median pixel feature intensity after background subtraction (EXPRESSION column) was log2 transformed, and normalized on the median for that hybridization (giving the VALUE column) to obtain the values given in the data table.
|
| |
|
| Submission date |
Aug 25, 2006 |
| Last update date |
Jun 12, 2013 |
| Contact name |
Amanda J. Moehring |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University
|
| Department |
DCMB/Biology
|
| Lab |
Mohamed Noor
|
| Street address |
Box 91000
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL3829 |
| Series (2) |
| GSE5607 |
Comparison of D. simulans/D. sechellia F1 male hybrid genome-wide expression values to pure-species parents |
| GSE5655 |
Comparison of pure-species Drosophila expression to hybrid expression |
|
