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Sample GSM13169 Query DataSets for GSM13169
Status Public on Feb 09, 2004
Title liver, 48h sugar, chip 1
Sample type RNA
 
Channel 1
Source name pool1, sugar
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool3 sugar = 3 animals (No. Su1, Su2 and Su3) fed before the experiment with standard food, ad libitum, during the 48h experiment with 50% (w/v) sugar solution (40% glucose, 10% sucrose).
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_054
 
Submission date Dec 03, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE851 liver, 48h sugar
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.896232404 100 58 0 56 0 1.505 80 384 91 63 0 90 218 0 218 0 1.546 52 309 508 418 0 100 1246 0 1105 0 1.535 80 396 4167 2199 0
A#10 100 66 0 222 0 1.804 80 333 58 163 -50 100 271 0 1383 0 1.897 80 333 351 1147 -50 100 1563 0 7985 0 2.056 80 333 2170 6712 -50
A#100 0.319077551 80 58 0 49 0 2 52 222 36 43 0 90 214 0 161 0 2.686 52 261 270 287 0 90 1257 0 750 0 2.132 52 247 1356 1639 0
A#1000 0.529568432 90 46 0 45 0 1.42 52 273 91 72 0 90 128 0 123 0 1.381 52 273 606 484 0 90 691 0 525 0 1.42 52 273 4263 2893 0
A#10000 0.419219752 110 54 0 54 0 1.245 80 254 170 135 0 110 171 0 190 0 1.23 80 265 1115 976 0 110 926 0 905 0 1.248 80 254 7814 5284 0
A#10001 0.140195314 100 51 0 54 0 1.302 80 249 136 125 0 100 169 0 181 0 1.281 80 237 877 900 0 100 890 0 871 0 1.272 80 237 6535 5029 0
A#10002 -0.301038769 90 54 0 56 0 1.313 52 240 115 139 0 100 185 0 189 0 1.329 80 265 844 1033 0 100 1026 0 905 0 1.422 80 265 6045 6025 0
A#10003 0.111937351 90 54 0 55 0 1.26 52 253 143 134 0 90 176 0 183 0 1.235 52 241 1007 990 0 90 1006 0 916 0 1.202 52 242 6877 5667 0
A#10004 -0.119165854 110 54 0 53 0 1.122 80 222 581 605 0 110 167 0 178 0 1.132 80 239 3872 4370 0 100 961 0 865 0 1.168 80 235 27375 23985 0
A#10005 0.213920946 110 54 0 54 0 1.237 80 215 210 185 0 110 167 0 174 0 1.212 80 238 1427 1341 0 110 1006 0 866 0 1.247 80 259 9799 7400 0
A#10006 0.483963608 110 56 0 53 0 1.199 80 264 463 343 0 110 157 0 171 0 1.152 80 259 3132 2532 0 110 954 0 852 0 1.244 80 273 21545 14203 0
A#10007 0.03210994 100 54 0 53 0 1.18 80 247 399 378 0 100 156 0 171 0 1.173 80 248 2653 2755 0 100 884 0 828 0 1.181 80 247 18026 14883 0
A#10008 -0.288520797 100 51 0 53 0 1.323 80 258 114 136 0 100 158 0 178 0 1.357 80 259 762 943 0 100 828 0 830 0 1.417 80 259 5194 5117 0
A#10009 0.244369414 80 51 0 52 0 1.58 52 222 54 53 0 80 150 0 167 0 1.426 52 222 385 387 0 80 782 0 814 0 1.518 52 222 2451 2074 0
A#1001 -0.219777636 100 47 0 45 0 2.299 80 347 29 42 0 100 127 0 124 0 2.738 80 347 197 265 0 100 681 0 547 0 2.6 80 347 1214 1595 0
A#10010 -0.025341276 90 52 0 51 0 1.176 52 281 379 383 0 90 153 0 163 0 1.177 52 281 2480 2550 0 90 805 0 761 0 1.224 52 285 17826 15259 0
A#10011 0.166029511 80 50 0 51 0 1.279 52 252 128 116 0 80 142 0 156 0 1.343 52 252 850 812 0 70 815 0 791 0 1.341 32 190 5698 4764 0
A#10012 -0.02697044 90 49 0 49 0 1.542 52 256 36 40 0 80 126 0 145 0 1.623 52 242 238 292 0 90 740 0 702 0 2.034 52 305 1326 1580 0
A#10013 -0.040638257 90 48 0 50 0 1.743 52 256 34 45 0 90 122 0 153 0 1.806 52 256 257 303 0 80 696 0 708 0 1.828 52 231 1712 1726 0
A#10014 0.10461027 90 49 0 51 0 1.33 52 270 137 136 0 90 137 0 158 0 1.301 52 282 951 939 0 90 736 0 713 0 1.321 52 270 6826 5362 0

Total number of rows: 43200

Table truncated, full table size 5618 Kbytes.




Supplementary data files not provided

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