Data table header descriptions |
ID_REF |
|
VALUE |
log2 ratio, loess normalized, multiplied with -1 (dye swap) |
DIA_L |
spot diameter, used as quality parameter |
CH1_BKG_L |
CH1 (B635) median background intensity, used in quality estimation |
CH1_SAT_L |
CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots |
CH2_BKG_L |
CH2 (B532) median background intensity, used in quality estimation |
CH2_SAT_L |
CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots |
RATIOS_SD_L |
standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter |
F_AREA_L |
number of foreground pixel for both channels |
B_AREA_L |
number of background pixel for both channels |
CH1_MEDIAN_L |
CH1 (F635-B635) median forground intensity minus median background intensity, used as basic value for calculations |
CH2_MEDIAN_L |
CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations |
FLAG_L |
quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis |
DIA_M |
spot diameter, used as quality parameter |
CH1_BKG_M |
CH1 (B635) median background intensity, used in quality estimation |
CH1_SAT_M |
CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots |
CH2_BKG_M |
CH2 (B532) median background intensity, used in quality estimation |
CH2_SAT_M |
CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots |
RATIOS_SD_M |
standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter |
F_AREA_M |
number of foreground pixel for both channels |
B_AREA_M |
number of background pixel for both channels |
CH1_MEDIAN_M |
CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations |
CH2_MEDIAN_M |
CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations |
FLAG_M |
quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis |
DIA_H |
spot diameter, used as quality parameter |
CH1_BKG_H |
CH1 (B635) median background intensity, used in quality estimation |
CH1_SAT_H |
CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots |
CH2_BKG_H |
CH2 (B532) median background intensity, used in quality estimation |
CH2_SAT_H |
CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots |
RATIOS_SD_H |
standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter |
F_AREA_H |
number of foreground pixel for both channels |
B_AREA_H |
number of background pixel for both channels |
CH1_MEDIAN_H |
CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations |
CH2_MEDIAN_H |
CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations |
FLAG_H |
quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis |