|
| Status |
Public on Feb 11, 2014 |
| Title |
Holstein Friesian_Peak lactation_sucject 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
peripheral blood nuclear cells
|
| Organism |
Bos taurus |
| Characteristics |
breed: Holstein-Fresian
physiological state: peak lactation
|
| Treatment protocol |
Blood collection into Rneasy Protect Animal Blood Tubes (Qiagen, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Rneasy Protect Animal Blood Kit following manufacturer's instructions (Qiagen, USA)
|
| Label |
Cy5
|
| Label protocol |
according to the manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
peripheral blood nuclear cells - common reference
|
| Organism |
Bos taurus |
| Characteristics |
sample type: peripheral blood nuclear cells from 20 bovine which were not included in the research groups
|
| Treatment protocol |
Blood collection into Rneasy Protect Animal Blood Tubes (Qiagen, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Rneasy Protect Animal Blood Kit following manufacturer's instructions (Qiagen, USA)
|
| Label |
Cy3
|
| Label protocol |
according to the manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
according to the manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Feb 07, 2014 |
| Last update date |
Feb 11, 2014 |
| Contact name |
Magdalena Łój |
| E-mail(s) |
[email protected]
|
| Organization name |
Warsaw University of Life Sciences
|
| Department |
Department of Physiological Sciences
|
| Street address |
Nowoursynowska 166
|
| City |
Warsaw |
| ZIP/Postal code |
02-787 |
| Country |
Poland |
| |
|
| Platform ID |
GPL11649 |
| Series (1) |
| GSE54790 |
The transcriptomic profile of peripheral blood nuclear cells in bovine and cervine in peak lactation and dry period |
|
