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Sample GSM13241 Query DataSets for GSM13241
Status Public on Feb 09, 2004
Title liver, 48h sugar, chip 4
Sample type RNA
 
Channel 1
Source name pool2, sugar
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 48h experiment with standard food, ad libitum; pool2 sugar = 3 animals (No. Su4, Su5 and Su6) fed before the experiment with standard food, ad libitum, during the 48h experiment with 50% (w/v) sugar solution (40% glucose, 10% sucrose).
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_059
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE851 liver, 48h sugar
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 100 45 0 85 0 3.531 80 411 5 58 -50 50 113 0 529 0 1.687 12 104 126 969 0 50 485 0 3079 0 1.823 12 99 967 5863 0
A#10 -0.153063846 80 44 0 41 0 2.694 52 214 25 57 0 80 96 0 97 0 3.144 52 229 111 364 0 80 439 0 444 0 2.233 52 229 1020 2015 0
A#100 60 47 0 40 0 2.756 32 164 7 19 0 100 108 0 88 0 4.076 80 300 26 91 -50 60 373 0 380 0 2.864 32 160 235 766 0
A#1000 0.575424638 90 50 0 38 0 2.754 52 292 16 27 0 90 119 0 69 0 2.355 52 296 99 154 0 90 501 0 252 0 3.206 52 302 561 926 0
A#10000 -0.012586937 100 51 0 42 0 1.476 80 253 143 152 0 100 139 0 96 0 1.393 80 247 775 1046 0 100 536 0 391 0 1.331 80 254 4655 5997 0
A#10001 -0.185105026 100 52 0 43 0 1.447 80 218 94 105 0 100 159 0 100 0 1.587 80 222 448 711 0 100 594 0 410 0 1.385 80 212 2887 4260 0
A#10002 0.003797323 100 58 0 46 0 1.55 80 237 71 79 0 100 175 0 121 0 1.728 80 236 386 537 0 100 690 0 512 0 1.63 80 215 2196 3008 0
A#10003 -0.293233229 90 60 0 47 0 1.608 52 256 75 93 0 90 188 0 127 0 1.632 52 232 393 653 0 90 802 0 589 0 1.561 52 253 2087 3685 0
A#10004 -0.397115801 110 60 0 45 0 1.183 80 305 527 699 0 110 190 0 114 0 1.176 80 296 2907 4758 0 110 789 0 510 0 1.167 80 285 17121 27772 0
A#10005 -0.096116632 110 61 0 45 0 1.479 80 318 91 105 0 110 213 0 115 0 1.592 80 319 514 731 0 110 837 0 521 0 1.432 80 285 3115 4246 0
A#10006 0.408911006 100 64 0 46 0 1.476 80 260 271 208 0 100 225 0 125 0 1.274 80 256 1610 1473 0 100 898 0 573 0 1.277 80 255 8681 8194 0
A#10007 -0.173962036 100 64 0 47 0 1.374 80 253 325 371 0 100 220 0 133 0 1.255 80 257 1784 2619 0 100 905 0 586 0 1.246 80 252 10333 14807 0
A#10008 -0.100250597 100 61 0 47 0 1.402 80 214 158 172 0 100 200 0 124 0 1.45 80 238 943 1255 0 100 874 0 551 0 1.51 80 236 5235 7111 0
A#10009 100 61 0 47 0 4.068 80 258 11 9 -50 100 201 0 119 0 4.129 80 279 43 59 -50 100 867 0 540 0 4.895 80 276 275 332 -50
A#1001 0.313729472 80 49 0 38 0 2.224 52 250 14 23 0 90 116 0 68 0 2.529 52 292 69 177 0 90 429 0 253 0 2.549 52 278 510 1028 0
A#10010 -0.126082073 90 63 0 47 0 1.439 52 261 240 273 0 90 208 0 128 0 1.249 52 257 1381 1912 0 90 889 0 564 0 1.267 52 271 8169 10690 0
A#10011 -0.170525625 70 63 0 47 0 1.469 32 200 86 94 0 80 201 0 123 0 1.723 52 229 417 619 0 70 877 0 579 0 1.509 32 200 3260 3842 0
A#10012 100 62 0 47 0 3.529 80 330 3 12 -50 100 201 0 117 0 3.224 80 301 14 98 -50 100 828 0 542 0 3.102 80 354 136 521 -50
A#10013 70 62 0 46 0 2.173 32 198 25 32 0 60 230 0 113 0 2.129 32 164 118 192 0 70 923 0 568 0 3.086 32 209 583 1097 0
A#10014 0.220387563 80 60 0 45 0 2.21 52 223 53 47 0 80 201 0 113 0 1.814 52 224 309 354 0 80 807 0 531 0 1.578 52 234 1632 1895 0

Total number of rows: 43200

Table truncated, full table size 5504 Kbytes.




Supplementary data files not provided

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