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Sample GSM13242 Query DataSets for GSM13242
Status Public on Feb 09, 2004
Title liver, 48h sugar, chip 5
Sample type RNA
 
Channel 1
Source name pool1 sugar
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 sugar = 3 animals (No. Su1, Su2 and Su3) fed before the experiment with standard food, ad libitum, during the 48h experiment with 50% (w/v) sugar solution (40% glucose, 10% sucrose).
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp8_010
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE851 liver, 48h sugar
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 100 45 0 43 0 3.283 80 398 19 13 0 100 122 0 118 0 4.14 80 410 102 91 0 50 818 0 686 0 2.171 12 74 517 848 0
A#10 -0.496921474 90 46 0 39 0 1.359 52 313 238 381 0 90 120 0 86 0 1.273 52 313 1295 2622 0 90 542 0 357 0 1.199 52 314 9909 15391 0
A#100 -0.184391721 80 49 0 47 0 1.901 52 256 44 54 0 80 141 0 142 0 1.828 52 230 219 364 0 80 648 0 667 0 1.531 52 246 1397 2273 0
A#1000 0.175337451 90 57 0 50 0 1.667 52 257 81 87 0 100 166 0 148 0 1.674 80 279 405 534 0 100 882 0 683 0 1.425 80 271 3143 3130 0
A#10000 0.432354622 90 84 0 49 0 1.483 52 257 124 89 0 90 348 0 147 0 1.452 52 281 697 627 0 90 1906 0 697 0 1.526 52 257 4753 3474 0
A#10001 0.144324741 90 79 0 48 0 1.386 52 256 116 101 0 90 330 0 142 0 1.4 52 257 707 707 0 90 1832 0 655 0 1.357 52 256 4234 4087 0
A#10002 -0.111119056 90 82 0 48 0 1.345 52 256 155 163 0 100 324 0 141 0 1.319 80 277 956 1154 0 90 1994 0 646 0 1.241 52 279 6185 6812 0
A#10003 -0.089728225 90 86 0 49 0 1.298 52 290 228 236 0 90 354 0 145 0 1.276 52 256 1321 1619 0 90 2048 0 677 0 1.365 52 278 9177 9152 0
A#10004 -0.328266588 100 82 0 48 0 1.167 80 302 514 576 0 100 328 0 137 0 1.167 80 287 2973 3971 0 100 1837 0 659 0 1.155 80 299 19868 22178 0
A#10005 -0.015769446 90 74 0 47 0 1.448 52 221 90 96 0 80 294 0 137 0 1.523 52 187 805 753 0 90 1731 0 599 0 1.403 52 220 3660 4230 0
A#10006 0.100789503 100 74 0 47 0 1.257 80 290 363 336 0 100 286 0 130 0 1.246 80 294 2231 2305 0 100 1621 0 583 0 1.18 80 306 13775 12900 0
A#10007 0.010875369 100 73 0 46 0 1.417 80 286 298 276 0 100 294 0 123 0 1.406 80 305 1725 1968 0 100 1556 1 551 2 1.379 80 315 10452 10856 0
A#10008 -0.02222737 90 67 0 45 0 1.185 52 243 258 266 0 100 248 0 118 0 1.269 80 250 1629 1845 0 90 1252 0 505 0 1.23 52 233 10546 10544 0
A#10009 -0.467866631 90 59 0 45 0 1.879 52 260 34 41 0 90 203 0 115 0 2.948 52 229 149 269 0 90 988 0 493 0 1.892 52 256 1199 1669 0
A#1001 -0.181318813 100 57 0 48 0 1.685 80 302 87 100 0 100 176 0 139 0 1.825 80 282 485 713 0 90 872 0 649 0 1.487 52 245 2928 4525 0
A#10010 -0.092530099 100 60 0 45 0 1.302 80 290 242 250 0 100 194 0 113 0 1.293 80 335 1439 1787 0 100 976 0 477 0 1.28 80 325 9682 9814 0
A#10011 -0.155090449 100 59 0 44 0 1.387 80 332 146 153 0 100 185 0 108 0 1.398 80 306 826 1062 0 100 944 0 460 0 1.281 80 314 5410 6014 0
A#10012 -0.019439778 90 56 0 44 0 1.81 52 268 70 66 0 100 180 0 108 0 2.066 80 276 360 428 0 90 920 0 460 0 1.59 52 256 2542 2711 0
A#10013 0.140943991 90 59 0 45 0 1.716 52 256 73 61 0 90 183 0 110 0 1.765 52 233 421 420 0 90 976 0 457 0 1.616 52 256 2345 2455 0
A#10014 0.339113162 90 63 0 45 0 1.616 52 294 100 73 0 100 205 0 110 0 1.521 80 335 543 492 0 90 1082 0 473 0 1.402 52 296 3478 3044 0

Total number of rows: 43200

Table truncated, full table size 5623 Kbytes.




Supplementary data files not provided

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