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Sample GSM13243 Query DataSets for GSM13243
Status Public on Feb 09, 2004
Title liver, 48h sugar, chip 6
Sample type RNA
 
Channel 1
Source name pool1 sugar
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 sugar = 3 animals (No. Su1, Su2 and Su3) fed before the experiment with standard food, ad libitum, during the 48h experiment with 50% (w/v) sugar solution (40% glucose, 10% sucrose).
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp8_011
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE851 liver, 48h sugar
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 100 76 0 48 0 3.467 80 418 7 23 -50 100 316 0 146 0 4.172 80 428 79 161 -50 100 1699 0 713 0 2.943 80 399 689 912 -50
A#10 -0.268306381 100 54 0 46 0 1.347 80 342 200 287 0 100 179 0 138 0 1.332 80 356 1186 2032 0 100 895 0 647 0 1.248 80 357 8488 11593 0
A#100 -0.03371555 90 54 0 49 0 2.064 52 256 35 47 0 80 169 0 157 0 2.406 52 210 192 322 0 80 805 0 776 0 2.057 52 210 1368 1830 0
A#1000 0.274986327 100 52 0 50 0 1.627 80 267 72 82 0 100 149 0 158 0 1.626 80 270 362 595 0 90 781 0 739 0 1.423 52 256 3795 3840 0
A#10000 0.278154132 100 70 0 50 0 1.513 80 279 92 111 0 100 258 0 144 0 1.56 80 282 570 789 0 100 1448 0 697 0 1.427 80 283 4216 4569 0
A#10001 -0.352515115 90 71 0 50 0 1.972 52 233 50 85 0 90 258 0 148 0 1.934 52 233 294 579 0 90 1419 0 714 0 1.974 52 234 1683 3343 0
A#10002 -0.179769098 100 72 0 50 0 1.567 80 273 117 175 0 100 261 0 150 0 1.389 80 273 650 1204 0 100 1600 0 740 0 1.401 80 268 4551 6554 0
A#10003 -0.298517901 100 75 0 50 0 1.38 80 265 140 233 0 100 274 0 147 0 1.353 80 262 882 1654 0 100 1645 0 736 0 1.334 80 265 6309 9173 0
A#10004 -0.265883221 100 77 0 48 0 1.21 80 270 335 487 0 100 289 0 143 0 1.259 80 254 1968 3555 0 100 1650 0 686 0 1.19 80 284 14877 20392 0
A#10005 -0.078628059 100 76 0 48 0 1.562 80 277 68 105 0 100 298 0 141 0 2.368 80 274 397 721 0 100 1590 0 673 0 1.591 80 310 3082 4077 0
A#10006 0.160780122 90 75 0 49 0 1.17 52 244 552 602 0 90 275 0 140 0 1.163 52 245 3247 4195 0 100 1601 0 650 0 1.191 80 273 21695 23412 0
A#10007 0.259509298 100 75 0 48 0 1.247 80 283 350 389 0 100 279 0 141 0 1.219 80 296 2121 2650 0 100 1608 0 643 0 1.247 80 276 14918 15651 0
A#10008 0.000424803 100 73 0 48 0 1.284 80 254 265 356 0 100 284 0 140 0 1.227 80 275 1688 2517 0 100 1528 0 671 0 1.213 80 262 11515 13779 0
A#10009 -0.009209708 90 71 0 49 0 1.656 52 257 68 91 0 90 268 0 142 0 1.577 52 278 367 631 0 90 1485 0 672 0 1.771 52 257 2729 3714 0
A#1001 -0.092544375 90 52 0 51 0 1.425 52 234 122 178 0 90 150 0 157 0 1.334 52 232 759 1252 0 90 766 0 753 0 1.283 52 256 5502 7308 0
A#10010 0.180842939 100 69 0 49 0 1.304 80 305 410 458 0 100 240 0 140 0 1.251 80 295 2500 3167 0 100 1389 0 631 0 1.227 80 312 17002 18567 0
A#10011 -0.239136039 100 68 0 48 0 1.875 80 261 67 102 0 100 237 0 137 0 1.841 80 281 374 702 0 110 1385 0 623 0 1.636 80 326 2536 4287 0
A#10012 -0.164677442 90 68 0 47 0 2.225 52 257 49 70 0 90 237 0 137 0 2.293 52 308 232 465 0 90 1314 0 589 0 1.782 52 258 1823 2848 0
A#10013 0.051735986 100 68 0 48 0 1.939 80 282 58 87 0 90 242 0 124 0 1.586 52 280 433 665 0 100 1381 0 573 0 1.692 80 297 2672 3352 0
A#10014 0.296692148 90 68 0 48 0 1.449 52 256 98 115 0 90 255 0 134 0 1.417 52 280 556 776 0 90 1366 0 607 0 1.478 52 256 4162 4382 0

Total number of rows: 43200

Table truncated, full table size 5596 Kbytes.




Supplementary data files not provided

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