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Sample GSM13244 Query DataSets for GSM13244
Status Public on Feb 09, 2004
Title liver, 48h sugar, chip 7
Sample type RNA
 
Channel 1
Source name pool1 normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, sugar
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 sugar = 3 animals (No. Su1, Su2 and Su3) fed before the experiment with standard food, ad libitum, during the 48h experiment with 50% (w/v) sugar solution (40% glucose, 10% sucrose).
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp8_012
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE851 liver, 48h sugar
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 80 46 0 50 0 3.01 52 268 16 36 0 70 167 0 154 0 4.781 32 212 152 273 0 70 445 0 384 0 2.389 32 206 807 808 0
A#10 0.340154599 100 45 0 41 0 1.55 80 373 210 483 0 90 142 0 90 0 1.285 52 316 2244 3578 0 100 369 0 200 0 1.328 80 365 7728 9209 0
A#100 -0.203229544 90 46 0 46 0 2.396 52 244 20 61 0 80 150 0 125 0 2.017 52 210 259 430 0 90 392 0 276 0 2.048 52 244 1003 1191 0
A#1000 -0.08422215 100 51 0 45 0 1.687 80 273 60 128 0 100 181 0 114 0 1.68 80 282 508 905 0 100 536 0 262 0 1.609 80 274 2009 2405 0
A#10000 0.33149436 100 50 0 44 0 1.484 80 275 114 243 0 100 195 0 112 0 1.681 80 278 1206 1738 0 100 482 0 249 0 1.561 80 275 3582 4446 0
A#10001 0.097740338 90 51 0 44 0 1.485 52 233 74 137 0 90 189 0 112 0 1.627 52 233 671 973 0 90 450 0 245 0 1.495 52 234 2607 2590 0
A#10002 -0.119059674 100 50 0 44 0 1.337 80 274 187 278 0 100 176 0 105 0 1.34 80 266 1726 2000 0 100 474 0 226 0 1.313 80 270 6465 5104 0
A#10003 0.417908623 100 48 0 43 0 1.372 80 276 146 306 0 100 159 0 100 0 1.429 80 250 1237 2136 0 100 480 0 216 0 1.426 80 247 4531 5610 0
A#10004 0.149705592 100 49 0 43 0 1.21 80 259 720 1183 0 100 179 0 105 0 1.244 80 243 7141 8324 0 100 480 0 217 0 1.176 80 249 24312 21385 0
A#10005 -0.115366398 100 49 0 43 0 1.505 80 256 88 151 0 100 188 0 104 0 1.516 80 223 926 1011 0 100 451 0 225 0 1.49 80 237 2939 2669 0
A#10006 0.151019332 100 51 0 44 0 1.228 80 235 568 932 0 100 188 0 101 0 1.242 80 247 5645 6547 0 100 464 0 215 0 1.208 80 252 19556 17350 0
A#10007 -0.023748393 100 50 0 44 0 1.232 80 271 503 751 0 100 191 0 98 0 1.249 80 270 5001 5176 0 100 453 0 209 0 1.202 80 275 16569 13782 0
A#10008 -0.370387834 100 49 0 43 0 1.296 80 258 287 365 0 100 173 0 93 0 1.317 80 254 2898 2503 0 100 390 0 197 0 1.267 80 261 9555 6527 0
A#10009 0.154193997 90 48 0 42 0 1.759 52 268 74 142 0 90 163 0 97 0 1.649 52 256 694 1022 0 90 439 0 196 0 1.455 52 244 2513 2713 0
A#1001 0.219136554 100 49 0 44 0 1.717 80 262 89 216 0 100 188 0 107 0 1.639 80 280 857 1566 0 100 512 0 230 0 1.583 80 262 3249 4256 0
A#10010 0.4710899 100 50 0 42 0 1.239 80 321 502 1080 0 100 179 0 97 0 1.259 80 335 4930 7213 0 100 431 0 196 0 1.2 80 319 18046 19457 0
A#10011 -0.414727137 100 49 0 43 0 1.588 80 296 100 139 0 110 180 0 95 0 1.536 80 317 1052 948 0 100 429 0 193 0 1.443 80 273 3587 2514 0
A#10012 -0.128874992 90 48 0 43 0 2.408 52 303 26 53 0 80 155 0 92 0 2.333 52 221 259 376 0 90 417 0 194 0 2.28 52 268 815 956 0
A#10013 0.146205619 90 49 0 42 0 2.805 52 267 16 42 0 90 167 0 92 0 2.542 52 257 172 296 0 90 410 0 191 0 2.395 52 257 705 827 0
A#10014 0.203730213 90 48 0 42 0 1.821 52 291 53 104 0 90 161 0 90 0 1.69 52 279 430 732 0 90 418 0 190 0 1.926 52 302 1693 1895 0

Total number of rows: 43200

Table truncated, full table size 5563 Kbytes.




Supplementary data files not provided

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