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Sample GSM13253 Query DataSets for GSM13253
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 4
Sample type RNA
 
Channel 1
Source name pool3, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool3, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool3 normal = 4 animals (No. N7, N8, N9 and N10) fed during and before the 48h experiment with standard food, ad libitum; pool3 starved = 4 animals (No. St7, St8, St9 and St10) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp6_030
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.342309467 100 51 0 57 0 1.538 80 392 87 60 0 100 166 0 223 0 1.456 80 392 542 413 0 100 729 0 1134 0 1.337 80 387 3397 2485 0
A#10 -0.196218838 100 45 0 47 0 1.298 80 321 564 540 0 100 119 0 135 0 1.252 80 309 3735 3792 0 100 496 0 658 0 1.232 80 331 20972 21057 0
A#100 0.958463872 100 75 0 86 0 1.508 80 224 114 59 0 100 305 0 407 0 1.408 80 220 753 430 0 100 1582 0 2078 0 1.497 80 231 4689 2597 0
A#1000 0.597057347 100 49 0 51 0 1.222 80 237 275 154 0 100 137 0 161 0 1.197 80 244 1842 1088 0 110 652 0 727 0 1.223 80 230 10902 5900 0
A#10000 -0.334995372 110 49 0 51 0 1.254 80 266 145 150 0 110 136 0 159 0 1.258 80 266 1032 1093 0 110 580 0 757 0 1.254 80 239 5801 5943 0
A#10001 0.394155749 110 48 0 51 0 1.228 80 255 188 135 0 110 132 0 167 0 1.2 80 268 1189 934 0 110 590 0 768 0 1.232 80 265 7023 5137 0
A#10002 0.486137671 110 49 0 51 0 1.15 80 231 346 245 0 110 129 0 163 0 1.148 80 237 2239 1703 0 110 590 0 787 0 1.139 80 249 12921 9475 0
A#10003 -0.30215658 110 48 0 50 0 1.128 80 252 397 414 0 110 124 0 153 0 1.124 80 252 2636 3010 0 110 544 0 749 0 1.138 80 252 15702 16524 0
A#10004 -0.179085321 120 47 0 50 0 1.139 120 240 646 627 0 120 121 0 150 0 1.154 120 228 4314 4426 0 120 519 0 719 0 1.138 120 228 24566 24769 0
A#10005 0.46800135 110 47 0 50 0 1.17 80 233 295 210 0 110 120 0 147 0 1.193 80 218 1991 1490 0 110 519 0 718 0 1.173 80 213 11065 8199 0
A#10006 0.231341047 110 47 0 49 0 1.114 80 227 811 656 0 110 123 0 147 0 1.103 80 238 5503 4724 0 110 520 0 695 0 1.089 80 218 31573 26152 0
A#10007 0.065863031 120 47 0 49 0 1.178 120 202 527 460 0 120 125 0 148 0 1.195 120 202 3441 3278 0 120 560 0 695 0 1.187 120 188 20297 18379 0
A#10008 -0.015578427 110 48 0 49 0 1.126 80 210 455 422 0 110 125 0 151 0 1.157 80 206 3104 2967 0 110 519 0 690 0 1.132 80 207 17485 16564 0
A#10009 0.18464068 110 47 0 49 0 1.261 80 246 129 103 0 110 125 0 147 0 1.251 80 234 944 753 0 110 450 0 686 0 1.231 80 243 5108 4240 0
A#1001 0.504410253 110 49 0 50 0 1.163 80 255 471 302 0 110 128 0 150 0 1.161 80 249 3213 2149 0 110 642 0 690 0 1.146 80 242 18464 12156 0
A#10010 -0.713598994 120 47 0 49 0 1.181 120 276 374 478 0 120 119 0 142 0 1.256 120 231 2573 3482 0 120 449 0 686 0 1.179 120 277 15132 19430 0
A#10011 -0.316418629 110 46 0 48 0 1.193 80 290 274 281 0 120 114 0 140 0 1.25 120 258 1724 1944 0 110 470 0 653 0 1.174 80 290 10640 11163 0
A#10012 0.27051449 110 46 0 49 0 1.302 80 290 129 102 0 110 109 0 144 0 1.322 80 266 932 713 0 110 507 0 646 0 1.303 80 276 5273 4067 0
A#10013 -0.033091161 110 48 0 50 0 1.22 80 271 208 187 0 110 116 0 146 0 1.241 80 271 1480 1331 0 110 514 0 685 0 1.196 80 258 7615 7409 0
A#10014 -0.03123837 110 49 0 50 0 1.175 80 271 238 212 0 110 121 0 146 0 1.175 80 271 1550 1474 0 110 523 0 697 0 1.142 80 272 9124 8449 0

Total number of rows: 43200

Table truncated, full table size 5761 Kbytes.




Supplementary data files not provided
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