|
| Status |
Public on Dec 31, 2015 |
| Title |
HOK(2) |
| Sample type |
RNA |
| |
|
| Source name |
normal human oral keratinocyte
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: oral keratinocyte
status: Healthy
smoking: No
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an RNeasy mini kit (Qiagen) according to the manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
500ng total RNA was converted into cDNA using biotinylated random nonamers, oligodeoxythymidine 18 primers and Illumina-supplied reagents, according to Illumina manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Data analysis was performed with GeneSpring GX software (Agilent Technologies). Arrays with less than 50% detected probes were excluded. Quantile normalisation was performed.
|
| |
|
| Submission date |
Feb 10, 2014 |
| Last update date |
Dec 31, 2015 |
| Contact name |
Chia Huei Lee |
| E-mail(s) |
[email protected]
|
| Organization name |
Taiwan National Health Research Institute
|
| Department |
National Institute of Cancer Research
|
| Street address |
35, Keyan Rd.
|
| City |
Zhunan Miaoli County |
| ZIP/Postal code |
114 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE54861 |
Gene expression profilings of normal human oral keratinocyte (HOK) and dysplastic oral keratinocyte cell line from a heavy smoker (DOK) |
|
