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Sample GSM13255 Query DataSets for GSM13255
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 6
Sample type RNA
 
Channel 1
Source name pool3, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool3, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool3 normal = 4 animals (No. N7, N8, N9 and N10) fed during and before the 48h experiment with standard food, ad libitum; pool3 starved = 4 animals (No. St7, St8, St9 and St10) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp6_032
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.264874384 110 41 0 42 0 1.347 80 454 113 82 0 110 102 0 113 0 1.415 80 453 816 600 0 110 136 0 115 0 1.458 80 451 708 447 0
A#10 -0.01576535 100 47 0 46 0 1.326 80 337 434 404 0 100 132 0 137 0 1.401 80 329 3150 2858 0 100 247 0 189 0 1.19 80 392 1974 2185 0
A#100 -0.35951505 100 62 0 63 0 2.911 80 276 26 17 -50 100 242 0 253 0 2.813 80 276 175 105 -50 80 288 0 255 0 1.385 52 199 554 372 0
A#1000 -0.683142403 110 53 0 52 0 1.216 80 261 244 144 0 110 176 0 178 0 1.24 80 261 1792 993 0 110 201 0 152 0 1.33 80 235 1061 610 0
A#10000 0.509705163 100 84 0 88 0 1.938 80 193 7 7 -50 100 389 0 429 0 2.453 80 196 40 63 -50 110 376 0 344 0 1.291 80 228 779 1032 0
A#10001 -0.646019378 100 84 0 90 0 2.396 80 229 24 34 0 90 401 0 429 0 2.972 52 231 183 287 0 100 363 0 320 0 1.286 80 253 828 705 0
A#10002 -0.450243799 100 88 0 100 0 2.422 80 246 24 53 0 90 449 0 508 0 2.818 52 229 129 310 0 110 311 0 295 0 1.236 80 270 1590 1342 0
A#10003 0.32374496 100 95 0 106 0 2.801 80 275 30 18 -50 100 461 0 556 0 2.523 80 223 214 155 -50 110 287 0 267 0 1.185 80 308 2044 2454 0
A#10004 0.101196872 120 100 0 110 0 1.25 120 232 292 318 0 120 509 0 588 0 1.242 120 207 2086 2085 0 110 257 0 251 0 1.112 80 287 5452 5860 0
A#10005 -0.185363842 100 99 0 108 0 2.026 80 176 36 56 -50 110 494 0 576 0 2.002 80 184 284 363 0 100 237 0 228 0 1.221 80 276 1285 1139 0
A#10006 -0.045759091 120 90 0 98 0 1.25 120 156 325 321 0 120 439 0 485 0 1.276 120 147 2324 2132 0 100 221 0 227 0 1.137 80 231 4244 4212 0
A#10007 0.183993 120 82 0 89 0 1.283 120 137 168 223 0 120 396 0 438 0 1.252 120 137 1277 1558 0 120 227 0 233 0 1.259 120 197 3109 3176 0
A#10008 0.249040668 120 83 0 89 0 1.368 120 161 180 234 0 120 384 0 442 0 1.36 120 161 1346 1717 0 110 230 0 233 0 1.222 80 199 2212 2440 0
A#10009 -0.121460751 100 83 0 92 0 3.186 80 181 -1 -2 -50 100 377 0 440 0 2.696 80 170 -11 -40 -50 100 228 0 231 0 1.362 80 235 641 568 0
A#1001 0.005509446 110 52 0 52 0 1.16 80 264 260 312 0 100 170 0 171 0 1.148 80 255 1848 2200 0 100 179 0 145 0 1.238 80 250 1783 1261 0
A#10010 -0.057863423 120 76 0 86 0 1.241 120 175 438 361 0 120 345 0 405 0 1.159 120 217 3150 2511 0 110 237 0 247 0 1.16 80 292 2590 3682 0
A#10011 0.929684413 120 74 0 81 0 1.838 120 253 73 51 0 120 324 0 374 0 1.952 120 257 481 348 0 110 237 0 256 0 1.237 80 324 1492 1839 0
A#10012 -0.234946335 50 74 0 83 0 1.723 12 79 28 21 0 100 319 0 346 0 3.415 80 226 21 95 -50 90 255 0 263 0 1.678 52 252 404 326 0
A#10013 -0.06092431 110 73 0 79 0 1.332 80 337 116 109 0 110 308 0 345 0 1.348 80 250 832 789 0 90 285 0 298 0 1.38 52 284 1202 1256 0
A#10014 -0.020667023 110 73 0 80 0 1.274 80 266 120 114 0 110 307 0 355 0 1.253 80 247 829 828 0 100 295 0 289 0 1.399 80 278 744 752 0

Total number of rows: 43200

Table truncated, full table size 5628 Kbytes.




Supplementary data files not provided

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