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Sample GSM13256 Query DataSets for GSM13256
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 7
Sample type RNA
 
Channel 1
Source name pool3, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool3, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool3 normal = 4 animals (No. N7, N8, N9 and N10) fed during and before the 48h experiment with standard food, ad libitum; pool3 starved = 4 animals (No. St7, St8, St9 and St10) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp6_033
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.338179482 110 44 0 43 0 1.745 80 453 104 48 0 110 120 0 114 0 1.52 80 453 748 330 0 110 541 0 513 0 1.677 80 453 4411 1887 0
A#10 -0.214624849 110 46 0 46 0 1.259 80 401 423 355 0 110 128 0 128 0 1.231 80 364 3071 2433 0 110 576 0 592 0 1.278 80 364 17798 13949 0
A#100 -1.29572035 100 80 0 71 0 1.656 80 304 140 42 0 100 378 0 305 0 1.481 80 303 1046 283 0 100 2214 0 1562 0 1.44 80 315 6145 1741 0
A#1000 -2.46410252 110 76 0 77 0 1.31 80 283 246 117 0 110 345 0 365 0 1.219 80 291 1835 820 0 110 1842 0 1881 0 1.227 80 298 10693 4396 0
A#10000 -0.598934028 110 88 0 87 0 1.278 80 237 167 112 0 110 418 0 431 0 1.305 80 191 1248 772 0 110 2449 0 2221 0 1.308 80 237 7492 4429 0
A#10001 -0.094040665 110 89 0 87 0 1.329 80 260 141 124 0 110 419 0 421 0 1.311 80 241 1041 834 0 110 2375 0 2200 0 1.282 80 245 6166 4884 0
A#10002 0.049153732 120 89 0 90 0 1.296 120 236 277 278 0 120 420 0 422 0 1.284 120 236 2042 1943 0 120 2297 0 2174 0 1.358 120 198 11902 10839 0
A#10003 -0.223688353 110 88 0 89 0 1.164 80 234 358 321 0 110 420 0 429 0 1.156 80 225 2684 2200 0 110 2307 0 2134 0 1.144 80 207 16185 12642 0
A#10004 0.052286214 120 88 0 89 0 1.254 120 237 779 813 0 120 414 0 433 0 1.185 120 202 5654 5639 0 120 2332 0 2168 0 1.331 120 202 33540 32222 0
A#10005 0.228441812 120 89 0 90 0 1.435 120 193 176 183 0 120 411 0 431 0 1.368 120 152 1212 1250 0 120 2338 0 2235 0 1.606 120 152 7548 7094 0
A#10006 0.009698765 120 94 0 90 0 1.5 120 177 606 618 0 120 433 0 431 0 1.225 120 153 4368 4297 0 120 2512 0 2300 0 1.393 120 153 26335 24487 0
A#10007 0.080062758 120 90 0 89 0 1.24 120 147 441 460 0 120 444 0 426 0 1.173 120 139 3178 3191 0 120 2488 0 2272 0 1.219 120 139 18859 17968 0
A#10008 0.164466543 120 85 0 86 0 1.297 120 157 366 394 0 120 406 0 403 0 1.252 120 145 2688 2826 0 120 2264 0 2144 0 1.252 120 149 15809 15456 0
A#10009 -0.024087769 100 83 0 83 0 1.433 80 200 91 82 0 110 374 0 395 0 1.378 80 192 680 566 0 100 2164 0 2048 0 1.398 80 196 3670 2905 0
A#1001 0.129257025 110 68 0 69 0 1.184 80 280 330 316 0 110 255 0 289 0 1.163 80 264 2411 2230 0 110 1480 0 1524 0 1.185 80 279 14248 12281 0
A#10010 -0.57885603 120 82 0 81 0 1.195 120 289 482 349 0 120 374 0 387 0 1.173 120 243 3518 2422 0 120 2184 0 1981 0 1.221 120 317 21028 13856 0
A#10011 -0.494630012 120 82 0 81 0 1.336 120 282 266 192 0 120 376 0 380 0 1.252 120 272 1922 1337 0 120 2026 0 1947 0 1.342 120 301 11362 7434 0
A#10012 -0.002713137 110 80 0 82 0 1.419 80 262 105 97 0 110 365 0 376 0 1.304 80 255 786 676 0 110 1927 0 1980 0 1.338 80 255 4706 3826 0
A#10013 -0.306197522 110 77 0 80 0 1.221 80 276 242 191 0 110 343 0 370 0 1.197 80 261 1673 1325 0 110 1919 0 1902 0 1.215 80 261 10240 7279 0
A#10014 -0.035622866 110 78 0 80 0 1.334 80 298 139 128 0 110 342 0 356 0 1.295 80 289 1036 914 0 110 1912 0 1876 0 1.307 80 289 6153 4760 0

Total number of rows: 43200

Table truncated, full table size 5768 Kbytes.




Supplementary data files not provided

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