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Sample GSM13257 Query DataSets for GSM13257
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 8
Sample type RNA
 
Channel 1
Source name pool3, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool3, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool3 normal = 4 animals (No. N7, N8, N9 and N10) fed during and before the 48h experiment with standard food, ad libitum; pool3 starved = 4 animals (No. St7, St8, St9 and St10) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp6_034
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.153450188 110 40 0 42 0 1.533 80 450 57 49 0 110 115 0 145 0 1.554 80 459 374 332 0 110 669 0 678 0 1.459 80 445 2612 1932 0
A#10 -0.339719468 110 44 0 46 0 1.195 80 399 458 374 0 110 125 0 154 0 1.181 80 369 2992 2731 0 110 808 0 745 0 1.195 80 369 22270 15393 0
A#100 100 91 0 83 0 1.852 80 238 85 42 -50 100 460 0 425 0 1.692 80 269 547 274 -50 100 3403 0 2372 0 1.919 80 300 3920 1620 -50
A#1000 -0.930560161 100 78 0 82 0 1.231 80 259 200 106 0 90 352 0 450 0 1.292 52 244 1319 674 0 100 2252 0 2041 0 1.265 80 227 9684 4349 0
A#10000 -0.484432451 110 64 0 72 0 1.21 80 215 197 140 0 110 277 0 333 0 1.225 80 206 1245 986 0 110 1762 0 1756 0 1.19 80 212 8923 5511 0
A#10001 0.002421548 110 66 0 72 0 1.275 80 253 143 136 0 110 276 0 352 0 1.266 80 230 929 960 0 110 1814 0 1824 0 1.25 80 223 6795 5410 0
A#10002 0.289328765 110 69 0 77 0 1.19 80 260 239 279 0 110 282 0 362 0 1.163 80 236 1614 1995 0 120 1876 0 1925 0 1.279 120 213 11360 11214 0
A#10003 -0.181540391 110 69 0 77 0 1.162 80 262 356 319 0 110 293 0 377 0 1.132 80 259 2330 2271 0 110 1911 0 1972 0 1.138 80 227 17616 12896 0
A#10004 0.193049542 120 68 0 76 0 1.243 120 204 855 991 0 120 283 0 372 0 1.164 120 219 5491 6919 0 120 1873 0 1896 0 1.149 120 223 40936 39560 0
A#10005 0.184541766 120 65 0 74 0 1.257 120 210 183 200 0 120 263 0 349 0 1.282 120 205 1154 1374 0 120 1770 0 1823 0 1.248 120 234 8500 7776 0
A#10006 -0.063833363 110 67 0 75 0 1.102 80 231 816 810 0 110 267 0 341 0 1.103 80 229 5259 5804 0 110 1913 0 1831 0 1.099 80 244 40019 32628 0
A#10007 0.200373442 120 67 0 73 0 1.215 120 200 479 557 0 120 268 0 334 0 1.161 120 203 3134 3859 0 120 1921 0 1788 0 1.131 120 198 22700 22066 0
A#10008 0.228258162 110 65 0 71 0 1.157 80 172 452 538 0 110 268 0 319 0 1.153 80 172 2950 3692 0 110 1829 0 1714 0 1.157 80 172 21846 21002 0
A#10009 -0.091379101 110 63 0 70 0 1.277 80 199 110 100 0 110 258 0 313 0 1.317 80 217 730 725 0 110 1743 0 1681 0 1.275 80 217 5122 3946 0
A#1001 0.045019203 100 71 0 80 0 1.191 80 259 271 266 0 100 310 0 414 0 1.187 80 273 1776 1871 0 100 2103 0 2158 0 1.206 80 256 13054 10366 0
A#10010 -0.635361303 120 64 0 68 0 1.188 120 254 657 462 0 120 255 0 302 0 1.151 120 261 4184 3233 0 120 1634 0 1616 0 1.162 120 256 31135 17953 0
A#10011 -0.538595605 120 64 0 69 0 1.473 120 284 185 128 0 120 250 0 303 0 1.384 120 292 1204 883 0 120 1602 0 1603 0 1.314 120 271 8364 5086 0
A#10012 0.184091677 110 62 0 66 0 1.37 80 280 88 96 0 110 243 0 292 0 1.387 80 251 570 688 0 110 1531 0 1523 0 1.303 80 257 4496 3747 0
A#10013 -0.008998118 110 62 0 65 0 1.388 80 280 92 93 0 120 251 0 285 0 1.771 120 207 555 567 0 120 1535 0 1469 0 1.455 120 221 4332 3251 0
A#10014 0.056868696 110 63 0 65 0 1.247 80 243 122 120 0 120 240 0 291 0 1.488 120 165 678 726 0 120 1593 0 1494 0 1.436 120 165 4781 4071 0

Total number of rows: 43200

Table truncated, full table size 5699 Kbytes.




Supplementary data files not provided

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