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Sample GSM13258 Query DataSets for GSM13258
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 9
Sample type RNA
 
Channel 1
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_020
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 100 55 0 69 0 2.456 80 389 16 20 -50 100 218 0 302 0 2.934 80 392 133 125 -50 50 1655 0 2125 0 2.361 12 72 1123 639 0
A#10 0.038781597 90 56 0 78 0 1.389 52 326 67 84 0 90 224 0 362 0 1.487 52 300 497 618 0 90 1250 0 1938 0 1.427 52 326 3062 3497 0
A#100 100 51 0 73 0 2.508 80 262 27 24 -50 100 183 0 316 0 2.534 80 258 178 166 -50 100 1107 0 1707 0 2.311 80 251 1246 875 -50
A#1000 0.750934487 100 57 0 77 0 1.58 80 285 58 56 0 100 217 0 336 0 1.556 80 285 457 352 0 90 1150 0 1864 0 1.546 52 270 3321 2168 0
A#10000 -0.078119608 100 56 0 83 0 1.373 80 209 79 118 0 100 201 0 382 0 1.271 80 204 648 804 0 100 1062 0 2113 0 1.362 80 216 3956 4718 0
A#10001 0.281683926 110 55 0 79 0 1.338 80 212 123 139 0 110 194 0 377 0 1.237 80 228 924 974 0 110 1046 0 1980 0 1.229 80 227 6256 5670 0
A#10002 0.384156739 110 56 0 83 0 1.238 80 255 175 183 0 110 205 0 376 0 1.201 80 245 1296 1302 0 110 1029 0 2079 0 1.221 80 227 8833 7445 0
A#10003 -0.141354051 110 55 0 83 0 1.339 80 263 132 191 0 110 222 0 380 0 1.287 80 282 969 1340 0 110 1068 0 2057 0 1.257 80 250 6521 7866 0
A#10004 0.043708258 110 55 0 79 0 1.166 80 245 483 578 0 110 206 0 373 0 1.125 80 246 3598 4042 0 100 1120 0 1944 0 1.126 80 242 23408 23330 0
A#10005 0.501114692 110 61 0 87 0 1.276 80 256 165 159 0 110 219 0 394 0 1.221 80 227 1210 1113 0 110 1307 0 2074 0 1.228 80 227 8422 6494 0
A#10006 -0.118993957 110 64 0 88 0 1.188 80 229 286 405 0 110 242 0 409 0 1.204 80 217 2237 2823 0 110 1425 0 2235 0 1.21 80 213 15198 16190 0
A#10007 0.062709892 110 63 0 84 0 1.157 80 232 332 400 0 110 242 0 397 0 1.143 80 222 2520 2773 0 110 1343 0 2167 0 1.151 80 201 16327 16221 0
A#10008 0.06723485 110 61 0 80 0 1.234 80 259 202 260 0 110 219 0 356 0 1.152 80 220 1482 1764 0 120 1255 0 2004 0 1.211 120 242 9697 9931 0
A#10009 50 78 0 94 0 1.635 12 92 41 42 0 100 196 0 316 0 1.868 80 237 284 358 -50 70 1611 0 2238 0 1.765 32 148 2118 1947 0
A#1001 0.306951915 100 56 0 73 0 1.461 80 258 139 164 0 100 186 0 322 0 1.466 80 258 1080 1102 0 100 1001 0 1744 0 1.302 80 273 6977 6268 0
A#10010 -0.314602132 110 59 0 79 0 1.157 80 336 298 471 0 110 183 0 322 0 1.17 80 292 2267 3342 0 110 1101 0 1858 0 1.164 80 357 16110 19063 0
A#10011 -0.0388097 110 53 0 76 0 1.319 80 271 149 205 0 110 182 0 330 0 1.28 80 266 1063 1413 0 110 983 0 1814 0 1.233 80 271 7603 8357 0
A#10012 0.484740208 100 53 0 84 0 1.356 80 224 97 88 0 100 192 0 376 0 1.316 80 197 662 626 0 100 1121 0 2144 0 1.396 80 203 4266 3467 0
A#10013 100 58 0 87 0 1.926 80 237 46 47 -50 100 209 0 388 0 1.564 80 204 294 352 -50 90 1252 0 2283 0 2.013 52 223 2093 1685 0
A#10014 -0.41170131 110 61 0 88 0 1.574 80 252 67 87 0 110 238 0 409 0 1.499 80 241 479 604 0 90 1443 0 2366 0 1.575 52 257 3700 3561 0

Total number of rows: 43200

Table truncated, full table size 5682 Kbytes.




Supplementary data files not provided

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