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Sample GSM13259 Query DataSets for GSM13259
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 10
Sample type RNA
 
Channel 1
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_021
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 100 47 0 63 0 3.152 80 382 7 20 -50 100 139 0 260 0 2.446 80 382 35 130 -50 100 698 0 1359 0 2.445 80 394 357 654 -50
A#10 -0.056442115 90 48 0 58 0 1.298 52 324 124 167 0 90 127 0 224 0 1.271 52 323 920 1144 0 90 709 0 1145 0 1.22 52 335 5934 6297 0
A#100 0.098685706 80 42 0 47 0 1.916 52 210 33 47 0 80 95 0 146 0 1.54 52 176 239 320 0 80 423 0 625 0 1.635 52 198 1639 1875 0
A#1000 0.505257782 100 47 0 51 0 1.76 80 274 72 68 0 100 123 0 168 0 1.496 80 274 496 481 0 100 629 0 819 0 1.414 80 305 3294 2510 0
A#10000 -0.17269742 110 42 0 43 0 1.297 80 221 98 134 0 110 91 0 107 0 1.258 80 230 652 948 0 110 386 0 440 0 1.29 80 259 4222 5463 0
A#10001 0.279578209 110 41 0 43 0 1.308 80 237 131 132 0 110 89 0 104 0 1.284 80 218 895 960 0 110 347 0 425 0 1.227 80 248 5874 5199 0
A#10002 0.351227801 110 43 0 43 0 1.248 80 235 203 188 0 110 93 0 101 0 1.197 80 209 1386 1320 0 110 396 0 437 0 1.188 80 211 9177 7548 0
A#10003 -0.237101985 110 43 0 44 0 1.654 80 219 55 84 0 110 94 0 106 0 1.733 80 219 360 573 0 120 454 0 459 0 1.683 120 181 2243 3256 0
A#10004 0.018100032 110 43 0 44 0 1.188 80 233 475 521 0 110 93 0 111 0 1.11 80 253 3199 3528 0 110 415 0 452 0 1.133 80 211 21038 20436 0
A#10005 0.359565071 100 43 0 44 0 1.295 80 225 169 160 0 100 93 0 111 0 1.233 80 225 1217 1110 0 100 414 0 473 0 1.259 80 226 7448 6298 0
A#10006 -0.148621862 120 44 0 44 0 1.617 120 190 267 323 0 120 97 0 109 0 1.305 120 194 1810 2321 0 120 374 0 475 0 1.32 120 187 11750 12999 0
A#10007 0.026816672 120 45 0 44 0 1.553 120 213 353 379 0 120 101 0 113 0 1.222 120 213 2439 2713 0 120 396 0 441 0 1.244 120 173 15566 15010 0
A#10008 0.010326906 110 44 0 44 0 1.293 80 250 137 165 0 110 103 0 116 0 1.233 80 264 963 1177 0 110 456 0 460 0 1.247 80 212 6340 6649 0
A#10009 0.309645853 100 43 0 44 0 1.541 80 264 58 59 0 110 99 0 110 0 1.462 80 275 361 428 0 100 415 0 453 0 1.416 80 260 2297 2480 0
A#1001 0.230658774 100 46 0 50 0 2.886 80 248 64 71 0 100 111 0 160 0 2.227 80 248 536 540 0 100 597 0 747 0 1.959 80 252 2713 2977 0
A#10010 -0.385669441 120 44 0 44 0 1.564 120 247 220 332 0 120 97 0 106 0 1.463 120 225 1550 2321 0 110 393 0 444 0 1.19 80 253 10680 13650 0
A#10011 -0.157018782 110 43 0 44 0 1.37 80 255 86 123 0 110 93 0 106 0 1.315 80 257 585 856 0 110 383 0 451 0 1.322 80 266 3848 4776 0
A#10012 0.245901295 110 43 0 44 0 1.558 80 273 56 69 0 110 91 0 109 0 1.443 80 308 377 447 0 110 367 0 450 0 1.455 80 257 2612 2571 0
A#10013 0.176258958 100 44 0 45 0 1.352 80 252 70 83 0 100 92 0 110 0 1.34 80 240 456 591 0 100 367 0 462 0 1.38 80 227 3260 3324 0
A#10014 0.01513713 100 44 0 44 0 1.531 80 236 106 119 0 110 94 0 111 0 1.343 80 243 761 963 0 110 375 0 471 0 1.379 80 237 4985 5766 0

Total number of rows: 43200

Table truncated, full table size 5600 Kbytes.




Supplementary data files not provided

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