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Sample GSM13260 Query DataSets for GSM13260
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 11
Sample type RNA
 
Channel 1
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_022
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.483586615 110 55 0 71 0 1.507 80 458 74 74 0 90 177 0 316 0 1.397 52 309 499 587 0 100 967 0 1656 0 1.36 80 375 3195 3114 0
A#10 -0.196082291 90 51 0 64 0 1.163 52 317 192 283 0 90 153 0 249 0 1.206 52 319 1356 1986 0 90 838 0 1347 0 1.24 52 319 9558 11401 0
A#100 0.165480266 100 51 0 62 0 1.928 80 247 38 51 0 90 149 0 241 0 1.669 52 216 299 346 0 90 851 0 1305 0 1.625 52 228 1756 1976 0
A#1000 0.472348611 110 49 0 50 0 1.464 80 318 76 87 0 110 132 0 153 0 1.438 80 312 564 590 0 110 706 0 745 0 1.628 80 312 3865 3400 0
A#10000 100 54 0 54 0 1.308 80 247 100 143 -50 100 169 0 189 0 1.287 80 261 642 1013 -50 100 889 0 755 0 1.29 80 249 4490 5888 -50
A#10001 100 52 0 50 0 1.252 80 256 160 161 -50 100 156 0 150 0 1.245 80 256 997 1157 -50 100 751 0 608 0 1.223 80 230 7250 6350 -50
A#10002 100 51 0 48 0 1.196 80 201 224 227 -50 100 143 0 132 0 1.176 80 201 1489 1586 -50 100 680 0 561 0 1.169 80 174 10361 8945 -50
A#10003 100 58 0 52 0 1.426 80 214 90 141 -50 100 154 0 156 0 1.541 80 201 591 968 -50 100 848 0 652 0 1.358 80 184 4572 5544 -50
A#10004 0.04179849 120 60 0 56 0 1.145 120 277 408 485 0 120 178 0 188 0 1.135 120 248 2736 3430 0 120 1017 0 886 0 1.133 120 232 19649 19215 0
A#10005 0.566926202 80 53 0 52 0 1.188 52 181 176 168 0 100 149 0 141 0 1.219 80 200 1054 1170 -50 100 819 0 671 0 1.305 80 184 7417 6616 -50
A#10006 -0.213416232 110 65 0 68 0 1.146 80 298 495 623 0 110 196 0 187 0 1.136 80 236 3143 4554 0 110 1419 0 1375 0 1.122 80 266 22160 24672 0
A#10007 0.274828915 90 86 0 90 0 1.147 52 241 289 308 0 90 311 0 363 0 1.197 52 232 1883 2289 0 90 1893 0 1974 0 1.154 52 232 13418 12376 0
A#10008 0.058709746 110 75 0 65 0 1.165 80 265 202 253 0 110 270 0 214 0 1.187 80 252 1352 1862 0 100 1637 0 1182 0 1.193 80 240 9031 10077 0
A#10009 100 62 0 54 0 1.899 80 264 39 46 -50 100 192 0 168 0 1.73 80 243 303 327 -50 100 1065 0 769 0 1.914 80 240 2026 1877 -50
A#1001 -0.080893193 110 48 0 49 0 1.682 80 251 51 73 0 110 120 0 147 0 1.575 80 261 368 536 0 110 617 0 706 0 1.533 80 257 2155 3167 0
A#10010 -0.431157087 110 64 0 58 0 1.495 80 283 157 261 0 110 223 0 195 0 1.392 80 255 1045 1953 0 100 1208 0 911 0 1.289 80 234 7837 10501 0
A#10011 100 71 0 62 0 2.592 80 253 22 75 -50 100 277 0 252 0 2.496 80 219 156 491 -50 100 1622 0 1131 0 2.153 80 215 1118 3157 -50
A#10012 100 67 0 62 0 2.027 80 244 47 52 -50 100 244 0 231 0 1.684 80 214 341 355 -50 100 1378 0 1063 0 1.627 80 207 2276 2187 -50
A#10013 100 76 0 75 0 2.018 80 239 33 49 -50 100 290 0 305 0 2.086 80 221 209 350 -50 100 1561 0 1537 0 2.439 80 221 1484 1944 -50
A#10014 100 116 0 117 0 2.351 80 281 42 80 -50 100 350 0 371 0 1.398 80 206 506 805 -50 100 2145 0 1825 0 1.361 80 206 3682 4784 -50

Total number of rows: 43200

Table truncated, full table size 5657 Kbytes.




Supplementary data files not provided

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