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Sample GSM13261 Query DataSets for GSM13261
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 12
Sample type RNA
 
Channel 1
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_023
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 100 50 0 60 0 2.772 80 389 2 3 -50 100 160 0 241 0 4.46 80 400 -3 49 -50 100 903 0 1230 0 4.373 80 412 88 332 -50
A#10 0.065467986 90 52 0 63 0 1.326 52 358 85 96 0 90 162 0 253 0 1.417 52 367 492 644 0 90 988 0 1294 0 1.434 52 302 3588 4002 0
A#100 80 50 0 59 0 1.898 52 199 23 26 0 70 171 0 249 0 3.17 32 168 144 141 0 60 1060 0 1313 0 2.44 32 148 1054 886 0
A#1000 0.792142484 100 57 0 52 0 1.382 80 292 104 72 0 100 192 0 167 0 1.319 80 302 647 526 0 100 1080 0 836 0 1.328 80 311 4410 2953 0
A#10000 -0.054997755 110 45 0 43 0 1.269 80 234 113 146 0 110 108 0 111 0 1.238 80 220 781 971 0 110 575 0 488 0 1.263 80 235 5141 5740 0
A#10001 0.293732201 110 45 0 44 0 1.28 80 229 127 120 0 110 109 0 115 0 1.272 80 246 771 828 0 110 596 0 498 0 1.247 80 271 5226 4699 0
A#10002 0.374757234 120 44 0 44 0 1.302 120 192 218 202 0 120 111 0 113 0 1.23 120 200 1452 1352 0 110 539 0 506 0 1.177 80 232 10386 7946 0
A#10003 -0.130378061 120 45 0 43 0 1.424 120 180 116 154 0 120 104 0 109 0 1.348 120 180 769 1086 0 120 492 0 487 0 1.353 120 196 5496 6007 0
A#10004 0.036477824 110 46 0 44 0 1.1 80 235 596 645 0 110 112 0 117 0 1.09 80 242 4003 4436 0 110 527 0 521 0 1.105 80 210 27812 25479 0
A#10005 0.347899242 110 47 0 45 0 1.191 80 241 207 188 0 110 116 0 119 0 1.185 80 243 1365 1332 0 110 513 0 545 0 1.171 80 224 9472 7690 0
A#10006 -0.042695286 120 46 0 46 0 1.298 120 185 289 325 0 120 102 0 117 0 1.238 120 192 2003 2371 0 120 497 0 531 0 1.213 120 184 14716 14217 0
A#10007 0.063546624 120 46 0 46 0 1.193 120 178 411 444 0 110 103 0 117 0 1.13 80 216 2866 3037 0 120 515 0 512 0 1.214 120 178 18691 17049 0
A#10008 0.087349576 110 45 0 45 0 1.149 80 178 361 373 0 110 104 0 116 0 1.135 80 232 2438 2546 0 110 480 0 516 0 1.146 80 193 16193 14801 0
A#10009 0.345392231 140 45 0 46 0 1.567 156 275 98 99 0 100 119 0 125 0 1.292 80 246 714 748 -50 130 495 0 552 0 1.551 120 243 4985 4284 0
A#1001 -0.393404625 100 57 0 50 0 2.044 80 267 59 77 0 100 179 0 157 0 2.031 80 268 433 538 0 100 1016 0 749 0 1.965 80 268 3476 3227 0
A#10010 -0.310790692 120 44 0 45 0 1.289 120 218 323 453 0 110 121 0 118 0 1.152 80 272 2349 3193 0 120 551 0 517 0 1.226 120 242 15412 17756 0
A#10011 -0.342057056 110 45 0 45 0 1.301 80 238 89 140 0 110 104 0 113 0 1.362 80 241 604 969 0 110 532 0 511 0 1.395 80 256 4095 5642 0
A#10012 0.34204999 110 46 0 45 0 1.323 80 237 93 93 0 110 103 0 115 0 1.363 80 237 606 662 0 110 460 0 509 0 1.274 80 241 4135 3666 0
A#10013 0.217065136 110 45 0 45 0 1.508 80 220 51 65 0 110 101 0 119 0 1.49 80 260 353 452 0 110 466 0 525 0 1.367 80 214 2494 2694 0
A#10014 -0.07134342 110 45 0 46 0 1.292 80 243 145 173 0 110 102 0 118 0 1.223 80 279 928 1208 0 110 485 0 502 0 1.292 80 226 6115 7060 0

Total number of rows: 43200

Table truncated, full table size 5618 Kbytes.




Supplementary data files not provided

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