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Sample GSM13262 Query DataSets for GSM13262
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 13
Sample type RNA
 
Channel 1
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_024
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.022612548 100 43 0 52 0 2.222 80 363 18 28 0 100 119 0 175 0 1.955 80 363 116 215 0 100 621 0 896 0 2.001 80 363 791 1209 0
A#10 0.052997328 110 52 0 62 0 1.426 80 440 128 201 0 120 158 0 229 0 1.719 120 461 673 920 0 110 1001 0 1265 0 1.538 80 399 6691 7931 0
A#100 -0.594968485 70 44 0 52 0 2.307 32 194 31 50 0 70 115 0 178 0 1.575 32 194 259 366 0 80 592 0 902 0 1.775 52 243 1434 1917 0
A#1000 0.175523853 100 44 0 49 0 1.47 80 273 65 113 0 90 117 0 148 0 1.396 52 280 463 782 0 90 599 0 679 0 1.387 52 280 3465 4691 0
A#10000 -0.303393608 110 42 0 42 0 1.386 80 275 87 104 0 110 96 0 104 0 1.28 80 258 631 720 0 110 470 0 449 0 1.299 80 270 4356 4128 0
A#10001 0.239023075 110 43 0 43 0 1.388 80 309 71 117 0 110 100 0 101 0 1.357 80 285 504 872 0 110 469 0 432 0 1.394 80 255 3574 4898 0
A#10002 0.353998981 110 42 0 42 0 1.216 80 220 147 263 0 120 99 0 100 0 1.339 120 221 1003 1774 0 120 451 0 436 0 1.315 120 198 6729 10206 0
A#10003 -0.356371554 110 43 0 43 0 1.589 80 226 60 72 0 110 98 0 103 0 1.574 80 211 446 576 0 110 456 0 433 0 1.637 80 198 3516 3340 0
A#10004 0.110067205 110 43 0 44 0 1.137 80 234 487 733 0 110 104 0 112 0 1.138 80 247 3544 5102 0 110 509 0 475 0 1.112 80 256 24428 29431 0
A#10005 0.191075349 110 43 0 44 0 1.303 80 230 81 138 0 110 95 0 104 0 1.235 80 224 637 992 0 110 581 0 486 0 1.324 80 267 3937 5504 0
A#10006 -0.617593919 120 42 0 43 0 1.279 120 200 256 236 0 120 85 0 94 0 1.281 120 186 1874 1593 0 120 427 0 381 0 1.306 120 193 12723 9776 0
A#10007 0.184912064 120 42 0 42 0 1.286 120 155 168 262 0 120 80 0 93 0 1.304 120 168 1219 1872 0 120 385 0 365 0 1.228 120 190 8049 10345 0
A#10008 0.238551744 120 43 0 42 0 1.314 120 163 198 345 0 120 82 0 91 0 1.304 120 183 1488 2445 0 120 401 0 383 0 1.285 120 225 9976 13629 0
A#10009 100 41 0 41 0 1.494 80 196 41 70 0 110 93 0 91 0 1.536 80 222 290 477 0 100 437 0 383 0 1.486 80 215 2073 2818 0
A#1001 0.188115475 100 44 0 48 0 1.311 80 276 181 285 0 100 116 0 143 0 1.305 80 284 1332 2085 0 100 568 0 666 0 1.325 80 284 9207 11753 0
A#10010 -0.529397902 120 42 0 43 0 1.165 120 286 359 345 0 120 92 0 98 0 1.197 120 294 2636 2439 0 120 472 0 407 0 1.175 120 298 17679 13784 0
A#10011 -0.615979521 110 42 0 42 0 1.282 80 222 117 117 0 110 85 0 95 0 1.323 80 222 848 786 0 110 403 0 397 0 1.295 80 231 6139 4711 0
A#10012 0.262995872 110 42 0 41 0 1.458 80 234 55 102 0 110 88 0 95 0 1.398 80 251 389 713 0 110 411 0 394 0 1.464 80 250 2692 4158 0
A#10013 0.167745697 110 42 0 42 0 1.935 80 209 23 47 0 110 85 0 94 0 1.932 80 226 193 322 0 110 400 0 398 0 1.822 80 218 1227 1856 0
A#10014 0.105412552 110 42 0 42 0 1.42 80 225 78 125 0 110 88 0 93 0 1.317 80 251 602 847 0 110 383 0 396 0 1.355 80 226 3603 4813 0

Total number of rows: 43200

Table truncated, full table size 5588 Kbytes.




Supplementary data files not provided

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