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Sample GSM13263 Query DataSets for GSM13263
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 14
Sample type RNA
 
Channel 1
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_025
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 -0.09650914 100 43 0 48 0 1.624 80 367 41 63 0 100 109 0 125 0 1.742 80 349 279 338 0 100 672 0 786 0 1.551 80 379 2220 2402 0
A#10 -0.093170559 90 44 0 47 0 1.247 52 356 151 205 0 90 112 0 119 0 1.199 52 303 1013 1070 0 90 677 0 731 0 1.257 52 307 8302 8235 0
A#100 -0.106902668 80 48 0 51 0 2.319 52 209 27 44 0 80 144 0 142 0 2.09 52 222 195 210 0 70 937 0 865 0 1.707 32 193 1298 1759 0
A#1000 -0.071484406 100 49 0 47 0 1.516 80 320 68 97 0 100 141 0 111 0 1.443 80 320 473 503 0 100 836 0 638 0 1.348 80 315 3747 3810 0
A#10000 -0.253014654 110 55 0 46 0 1.324 80 247 102 115 0 110 172 0 108 0 1.309 80 251 663 601 0 110 1018 0 616 0 1.287 80 223 5234 4584 0
A#10001 0.28973618 110 57 0 46 0 1.363 80 210 71 114 0 110 179 0 107 0 1.568 80 221 490 630 0 110 1081 0 618 0 1.448 80 219 3509 4565 0
A#10002 0.091242381 110 54 0 46 0 1.25 80 238 170 227 0 110 167 0 106 0 1.236 80 203 1141 1252 0 110 1105 0 614 0 1.211 80 236 8079 9196 0
A#10003 -0.300092536 110 53 0 45 0 1.363 80 262 116 129 0 110 152 0 102 0 1.269 80 242 735 679 0 110 877 0 562 0 1.295 80 254 6653 5222 0
A#10004 -0.022211634 110 51 0 45 0 1.214 80 212 290 367 0 110 148 0 101 0 1.17 80 257 1916 1932 0 110 884 0 557 0 1.167 80 261 14554 14836 0
A#10005 0.159371651 120 49 0 45 0 1.452 120 204 107 168 0 110 141 0 102 0 1.238 80 231 857 982 0 110 829 0 578 0 1.238 80 238 6313 7259 0
A#10006 -0.464343648 110 50 0 46 0 1.133 80 216 567 532 0 120 138 0 100 0 1.214 120 204 3608 2704 0 120 761 0 570 0 1.212 120 211 27626 19936 0
A#10007 -0.007340046 110 52 0 46 0 1.181 80 248 292 365 0 110 138 0 100 0 1.157 80 218 1983 2010 0 110 782 0 553 0 1.174 80 216 14194 15028 0
A#10008 0.244407864 110 52 0 45 0 1.234 80 264 155 247 0 110 138 0 100 0 1.234 80 263 1071 1268 0 110 784 0 551 0 1.213 80 246 7583 9354 0
A#10009 0.013703938 90 51 0 45 0 1.717 52 220 47 74 0 90 142 0 100 0 1.577 52 245 289 373 0 100 715 0 528 0 1.492 80 227 2481 2468 0
A#1001 0.194150975 100 51 0 47 0 1.604 80 282 72 109 0 100 148 0 111 0 1.931 80 298 373 522 0 100 851 0 677 0 1.766 80 268 3866 4700 0
A#10010 -0.541652001 120 51 0 47 0 1.197 120 317 305 274 0 110 137 0 99 0 1.181 80 259 2058 1508 0 120 735 0 542 0 1.208 120 217 16168 10985 0
A#10011 -0.420219089 110 49 0 46 0 1.245 80 280 167 168 0 110 132 0 99 0 1.229 80 248 1049 866 0 110 718 0 526 0 1.19 80 221 8749 6558 0
A#10012 0.120268493 110 47 0 44 0 1.344 80 270 61 96 0 110 118 0 94 0 1.412 80 263 433 510 0 110 665 0 499 0 1.352 80 268 3322 3813 0
A#10013 -0.214783617 100 47 0 44 0 1.439 80 246 59 76 0 110 110 0 93 0 1.512 80 247 422 380 0 110 690 0 499 0 1.358 80 257 2786 2947 0
A#10014 0.021390373 110 48 0 45 0 1.803 80 279 97 118 0 110 132 0 96 0 1.614 80 304 575 639 0 110 736 0 521 0 1.481 80 273 4307 4887 0

Total number of rows: 43200

Table truncated, full table size 5597 Kbytes.




Supplementary data files not provided

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