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Sample GSM13265 Query DataSets for GSM13265
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 16
Sample type RNA
 
Channel 1
Source name pool1, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool1, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool1 normal = 3 animals (No. N1, N2 and N3) fed during and before the 48h experiment with standard food, ad libitum; pool1 starved = 3 animals (No. St1, St2 and St3) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_027
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 100 47 0 63 0 3.134 80 389 5 2 -50 100 114 0 166 0 3.87 80 371 41 13 -50 100 567 0 972 0 2.999 80 385 204 198 -50
A#10 -0.387100125 80 44 0 60 0 1.71 52 274 40 36 0 90 103 0 149 0 1.798 52 325 254 154 0 80 483 0 896 0 1.691 52 276 1395 1045 0
A#100 70 44 0 56 0 3.508 32 209 13 15 0 60 98 0 130 0 2.991 32 159 78 76 0 70 476 0 765 0 2.758 32 202 440 492 0
A#1000 0.344357656 90 46 0 56 0 2.162 52 272 30 63 0 90 101 0 129 0 1.943 52 294 139 260 0 90 478 0 725 0 1.791 52 294 1080 1797 0
A#10000 -0.238871529 110 43 0 51 0 1.524 80 242 60 68 0 100 85 0 104 0 1.383 80 249 367 302 0 110 350 0 552 0 1.424 80 213 2505 2171 0
A#10001 0.062776439 100 43 0 50 0 1.562 80 227 60 88 0 110 84 0 104 0 1.488 80 231 336 374 0 100 306 0 546 0 1.456 80 194 2308 2600 0
A#10002 0.085972325 110 43 0 51 0 1.391 80 220 96 126 0 110 87 0 106 0 1.36 80 216 547 540 0 110 358 0 572 0 1.319 80 206 3374 3892 0
A#10003 -0.334738028 110 44 0 52 0 1.295 80 229 134 117 0 110 90 0 107 0 1.296 80 225 774 522 0 110 365 0 589 0 1.301 80 231 4819 3598 0
A#10004 -0.320749784 110 45 0 50 0 1.246 80 241 264 245 0 110 90 0 103 0 1.19 80 247 1465 979 0 110 364 0 552 0 1.189 80 240 9516 7155 0
A#10005 0.077865158 110 44 0 49 0 1.39 80 250 71 100 0 110 90 0 101 0 1.431 80 259 412 418 0 110 333 0 514 0 1.335 80 242 2592 3078 0
A#10006 -0.282803757 110 44 0 50 0 1.243 80 263 318 303 0 110 88 0 101 0 1.24 80 270 1817 1289 0 110 342 0 515 0 1.204 80 256 12091 9249 0
A#10007 0.021705302 110 44 0 50 0 1.313 80 257 163 203 0 110 89 0 102 0 1.314 80 266 949 901 0 110 375 0 517 0 1.261 80 252 6256 6374 0
A#10008 0.160917828 110 43 0 50 0 1.381 80 245 100 140 0 110 84 0 102 0 1.362 80 275 567 619 0 110 325 0 515 0 1.304 80 265 3617 4368 0
A#10009 -0.069949491 100 43 0 49 0 1.947 80 243 26 37 0 90 80 0 101 0 1.726 52 234 146 171 0 90 317 0 519 0 1.93 52 234 898 1218 0
A#1001 0.298240977 100 46 0 57 0 2.443 80 324 32 54 0 90 98 0 141 0 2.459 52 271 184 258 0 90 438 0 776 0 1.899 52 271 1317 2063 0
A#10010 -0.491115855 110 43 0 49 0 1.296 80 278 223 164 0 120 81 0 99 0 1.385 120 271 1116 677 0 120 341 0 519 0 1.284 120 312 7455 4770 0
A#10011 -0.296388645 110 43 0 50 0 1.361 80 294 117 108 0 110 81 0 100 0 1.343 80 258 668 453 0 110 343 0 518 0 1.326 80 265 4017 3243 0
A#10012 0.175266411 110 42 0 49 0 2.044 80 258 18 51 0 100 81 0 100 0 2.567 80 253 107 207 0 100 335 0 499 0 2.135 80 252 640 1493 0
A#10013 -0.181683772 110 42 0 49 0 2.427 80 275 10 35 0 110 84 0 100 0 2.29 80 256 53 143 0 110 331 0 503 0 2.615 80 260 308 974 0
A#10014 0.210872638 110 43 0 49 0 1.918 80 295 20 56 0 100 85 0 100 0 2.038 80 255 108 223 0 110 322 0 506 0 1.963 80 278 658 1562 0

Total number of rows: 43200

Table truncated, full table size 5532 Kbytes.




Supplementary data files not provided

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