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Sample GSM13266 Query DataSets for GSM13266
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 17
Sample type RNA
 
Channel 1
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 48h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_028
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.119986903 100 39 0 48 0 1.716 80 374 41 66 0 100 77 0 128 0 1.673 80 386 249 335 0 100 360 0 991 0 1.647 80 374 1933 3370 0
A#10 0.038781597 130 43 0 47 0 2.144 120 570 96 63 0 130 96 0 123 0 2.196 120 570 686 339 0 120 512 0 828 0 1.958 120 507 5174 2786 0
A#100 70 41 0 47 0 1.801 32 191 49 51 0 70 91 0 124 0 1.732 32 192 306 308 0 70 500 0 831 0 1.594 32 186 2315 2563 0
A#1000 0.463542719 100 46 0 49 0 1.4 80 281 113 101 0 100 111 0 122 0 1.427 80 275 748 587 0 100 562 0 854 0 1.386 80 281 5743 5348 0
A#10000 -0.039914062 120 47 0 52 0 1.384 120 156 151 203 0 120 127 0 145 0 1.56 120 168 1025 1178 0 110 686 0 1030 0 1.249 80 200 7843 11302 0
A#10001 0.327713331 110 48 0 52 0 1.205 80 215 199 200 0 110 127 0 142 0 1.24 80 221 1287 1174 0 110 698 0 981 0 1.224 80 250 9990 10801 0
A#10002 0.435741623 110 49 0 53 0 1.12 80 258 362 333 0 110 146 0 153 0 1.17 80 288 2400 1916 0 110 793 0 1059 0 1.162 80 259 17833 17409 0
A#10003 -0.34473628 110 50 0 53 0 1.925 80 231 62 117 0 110 159 0 152 0 1.584 80 262 394 621 0 110 780 0 1056 0 1.566 80 225 2966 5336 0
A#10004 -0.237265605 110 50 0 54 0 1.146 80 255 476 665 0 110 143 0 149 0 1.14 80 253 3385 3778 0 110 755 0 1008 0 1.135 80 209 23878 34574 0
A#10005 0.124571835 110 51 0 55 0 1.445 80 241 100 122 0 100 140 0 146 0 1.291 80 233 631 738 0 120 783 0 1015 0 1.754 120 203 4688 6072 0
A#10006 -0.016767712 120 52 0 54 0 1.179 120 181 674 800 0 110 134 0 150 0 1.167 80 219 4729 4659 0 110 828 0 1155 0 1.165 80 199 34066 42005 0
A#10007 -0.030712796 110 52 0 55 0 1.097 80 211 590 695 0 110 140 0 161 0 1.133 80 248 3759 3834 0 110 881 0 1144 0 1.138 80 244 28652 35332 0
A#10008 0.041703877 110 51 0 54 0 1.169 80 292 365 443 0 110 151 0 156 0 1.185 80 293 2452 2426 0 110 821 0 1039 0 1.151 80 252 18217 23512 0
A#10009 0.31600223 90 51 0 54 0 1.32 52 239 75 91 0 100 147 0 146 0 1.599 80 255 525 505 0 100 757 0 1006 0 1.412 80 231 3904 4585 0
A#1001 0.301467659 110 44 0 48 0 1.624 80 294 101 90 0 100 104 0 121 0 1.694 80 289 531 560 0 100 511 0 836 0 1.642 80 290 4468 4828 0
A#10010 -0.541430241 120 50 0 53 0 1.266 120 270 408 677 0 120 126 0 135 0 1.399 120 237 2710 3822 0 120 675 0 972 0 1.365 120 201 19760 35292 0
A#10011 -0.689833026 110 48 0 52 0 1.903 80 207 41 102 0 110 118 0 134 0 1.792 80 206 277 588 0 110 625 0 981 0 1.736 80 196 1916 5153 0
A#10012 0.251929295 110 47 0 50 0 1.36 80 230 100 119 0 110 110 0 125 0 1.4 80 237 690 670 0 110 627 0 863 0 1.361 80 237 4887 6081 0
A#10013 -0.506565647 100 46 0 50 0 1.994 80 250 19 54 0 100 108 0 127 0 1.741 80 259 140 300 0 100 580 0 806 0 1.874 80 217 969 3006 0
A#10014 0.20410481 110 46 0 50 0 1.265 80 274 186 218 0 110 112 0 135 0 1.469 80 257 1217 1133 0 120 570 0 845 0 1.663 120 210 6996 8527 0

Total number of rows: 43200

Table truncated, full table size 5689 Kbytes.




Supplementary data files not provided

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