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Sample GSM13267 Query DataSets for GSM13267
Status Public on Feb 09, 2004
Title liver, 48h starved, chip 18
Sample type RNA
 
Channel 1
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 48h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_029
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 0.457042478 90 43 0 49 0 1.946 52 325 36 44 0 100 112 0 131 0 2.134 80 391 184 235 0 100 547 0 669 0 1.872 80 391 1250 1671 0
A#10 -0.046871476 80 42 0 46 0 1.268 52 264 106 160 0 80 96 0 119 0 1.263 52 264 721 886 0 80 479 0 548 0 1.295 52 264 4909 5800 0
A#100 70 45 0 53 0 2.209 32 199 34 46 0 70 124 0 139 0 1.65 32 199 258 252 0 70 648 0 716 0 1.525 32 192 1715 1749 0
A#1000 0.512617982 90 48 0 52 0 1.238 52 282 129 115 0 90 115 0 133 0 1.227 52 270 862 641 0 90 603 0 664 0 1.261 52 261 5746 4094 0
A#10000 -0.013433255 110 47 0 49 0 1.269 80 216 127 187 0 110 116 0 128 0 1.22 80 208 860 1074 0 110 553 0 606 0 1.28 80 208 5812 6629 0
A#10001 0.40083596 110 47 0 49 0 1.253 80 222 154 165 0 110 118 0 124 0 1.267 80 240 1073 953 0 110 538 0 587 0 1.288 80 233 7000 5862 0
A#10002 0.340182265 110 46 0 49 0 1.189 80 220 325 329 0 110 114 0 121 0 1.277 80 237 2071 1788 0 110 517 0 572 0 1.161 80 208 14510 11574 0
A#10003 -0.095807434 100 47 0 49 0 1.345 80 245 108 171 0 100 117 0 121 0 1.366 80 240 764 972 0 100 559 0 576 0 1.316 80 229 4988 6155 0
A#10004 -0.072068901 110 47 0 50 0 1.152 80 246 690 940 0 110 114 0 122 0 1.172 80 236 4576 5344 0 110 551 0 619 0 1.162 80 250 31182 33426 0
A#10005 0.400328203 110 47 0 49 0 1.197 80 255 250 244 0 110 112 0 123 0 1.191 80 249 1659 1374 0 110 561 0 623 0 1.201 80 255 11061 8457 0
A#10006 0.041079225 110 47 0 50 0 1.127 80 260 670 837 0 110 117 0 125 0 1.124 80 260 4432 4735 0 110 570 0 616 0 1.133 80 266 30336 29693 0
A#10007 0.117800924 110 50 0 52 0 1.122 80 247 606 694 0 110 126 0 137 0 1.15 80 242 3921 4015 0 110 577 0 660 0 1.156 80 256 26188 24334 0
A#10008 0.075582073 110 47 0 51 0 1.166 80 246 278 357 0 110 112 0 134 0 1.173 80 227 1936 1967 0 110 564 0 640 0 1.175 80 223 12592 12611 0
A#10009 0.551891031 100 46 0 49 0 1.396 80 240 72 79 0 100 106 0 127 0 1.431 80 245 489 443 0 100 526 0 531 0 1.375 80 222 3280 2713 0
A#1001 0.345699997 90 46 0 51 0 1.167 52 290 318 302 0 90 113 0 134 0 1.167 52 290 2141 1736 0 90 605 0 661 0 1.158 52 290 14194 10716 0
A#10010 -0.45786745 110 46 0 48 0 1.121 80 265 381 718 0 110 109 0 122 0 1.134 80 273 2564 3997 0 110 508 0 512 0 1.128 80 254 17359 25017 0
A#10011 -0.342515325 110 47 0 49 0 1.293 80 276 135 253 0 110 105 0 123 0 1.308 80 272 920 1394 0 110 506 0 538 0 1.251 80 270 6236 9261 0
A#10012 0.224188471 110 47 0 48 0 1.296 80 259 114 138 0 110 102 0 120 0 1.259 80 259 747 785 0 110 478 0 552 0 1.264 80 275 5178 4755 0
A#10013 -0.260032016 110 46 0 49 0 1.956 80 257 28 67 0 110 104 0 119 0 1.972 80 270 180 362 0 110 473 0 542 0 1.817 80 272 1084 2462 0
A#10014 0.146594238 110 46 0 49 0 1.428 80 263 94 132 0 110 106 0 119 0 1.506 80 274 632 685 0 110 460 0 553 0 1.472 80 264 5102 4962 0

Total number of rows: 43200

Table truncated, full table size 5615 Kbytes.




Supplementary data files not provided

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