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Sample GSM13271 Query DataSets for GSM13271
Status Public on Jun 01, 2004
Title liver, 48h starved, chip 22
Sample type RNA
 
Channel 1
Source name pool2, normal
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name pool2, starved
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Total RNA and polyA RNA were prepared for each animal separately, and equal amounts of polyA RNA from each batch were pooled. Pool2 normal = 3 animals (No. N4, N5 and N6) fed during and before the 48h experiment with standard food, ad libitum; pool2 starved = 3 animals (No. St4, St5 and St6) fed before the experiment with standard food, ad libitum, starved during the 48h experiment.
The microarray was scanned 3 times: low scan (suffix _L in data table) with a low amplification / intensity to avoid saturated spots for proper data analysis, high scan (suffix _H in data table) with a high amplification / intensity to detect also weak fluorescent spots which are missed in the low scan and medium scan which lies between high and low intensity and gives additional data points for subsequent calculations. For additional information see publication and web link.
Keywords = nutrient response
Keywords = starvation
Keywords = liver
Keywords = anti-aging
Keywords = longevity
Lot batch = mp7_033
 
Submission date Dec 04, 2003
Last update date May 28, 2005
Contact name Michael J. Pankratz
E-mail(s) [email protected]
Phone ++49 +7247 82 6087
Fax ++49 +7247 82 3354
URL http://www.fzk.de/microarray
Organization name Forschungszentrum Karlsruhe
Department Institute of Genetics
Lab Pankratz
Street address P.O. Box 3640
City Karlsruhe
ZIP/Postal code 76021
Country Germany
 
Platform ID GPL765
Series (2)
GSE852 liver, 48h starved
GSE858 Mouse feeding experiment

Data table header descriptions
ID_REF
VALUE log2 ratio, loess normalized, multiplied with -1 (dye swap)
DIA_L spot diameter, used as quality parameter
CH1_BKG_L CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_L CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_L CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_L CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_L standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_L number of foreground pixel for both channels
B_AREA_L number of background pixel for both channels
CH1_MEDIAN_L CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_L CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_L quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_M spot diameter, used as quality parameter
CH1_BKG_M CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_M CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_M CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_M CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_M standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_M number of foreground pixel for both channels
B_AREA_M number of background pixel for both channels
CH1_MEDIAN_M CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_M CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_M quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis
DIA_H spot diameter, used as quality parameter
CH1_BKG_H CH1 (B635) median background intensity, used in quality estimation
CH1_SAT_H CH1 (F635) percentage of saturated foreground pixel, used to discriminate saturated spots
CH2_BKG_H CH2 (B532) median background intensity, used in quality estimation
CH2_SAT_H CH1 (F532) percentage of saturated foreground pixel, used to discriminate saturated spots
RATIOS_SD_H standard deviation of pixel to pixel linear ratio (CH1/CH2) (F635/F535), used as a quality parameter
F_AREA_H number of foreground pixel for both channels
B_AREA_H number of background pixel for both channels
CH1_MEDIAN_H CH1 (F635-B635) median foreground intensity minus median background intensity, used as basic value for calculations
CH2_MEDIAN_H CH2 (F532-B532) median foreground intensity minus median background intensity, used as basic value for calculations
FLAG_H quality flag of picture analysis software, if flag < 0 the spot is discarded from subsequent analysis

Data table
ID_REF VALUE DIA_L CH1_BKG_L CH1_SAT_L CH2_BKG_L CH2_SAT_L RATIOS_SD_L F_AREA_L B_AREA_L CH1_MEDIAN_L CH2_MEDIAN_L FLAG_L DIA_M CH1_BKG_M CH1_SAT_M CH2_BKG_M CH2_SAT_M RATIOS_SD_M F_AREA_M B_AREA_M CH1_MEDIAN_M CH2_MEDIAN_M FLAG_M DIA_H CH1_BKG_H CH1_SAT_H CH2_BKG_H CH2_SAT_H RATIOS_SD_H F_AREA_H B_AREA_H CH1_MEDIAN_H CH2_MEDIAN_H FLAG_H
A#1 90 37 0 45 0 1.494 52 324 49 68 0 90 67 0 110 0 1.646 52 336 325 350 0 90 283 0 597 0 1.48 52 336 2153 2471 0
A#10 0.077772239 80 38 0 45 0 1.289 52 258 197 250 0 80 73 0 106 0 1.274 52 254 1306 1280 0 80 258 0 576 0 1.236 52 246 8404 9410 0
A#100 60 40 0 48 0 2.041 32 166 14 33 0 70 90 0 122 0 1.841 32 198 122 191 0 70 434 0 714 0 1.638 32 190 1019 1369 0
A#1000 0.170114942 100 45 0 54 0 1.497 80 310 54 86 0 100 106 0 134 0 1.497 80 313 391 447 0 100 505 0 828 0 1.532 80 310 2953 3305 0
A#10000 -0.346074124 110 62 0 72 0 1.266 80 275 125 124 0 110 223 0 238 0 1.213 80 263 808 651 0 110 1320 0 1559 0 1.276 80 290 5487 4725 0
A#10001 0.220340493 100 62 0 72 0 1.369 80 282 96 151 0 100 219 0 234 0 1.287 80 240 645 738 0 100 1357 0 1500 0 1.3 80 291 4518 5573 0
A#10002 0.308651399 110 68 0 79 0 1.193 80 284 214 334 0 110 255 0 256 0 1.173 80 234 1437 1788 0 100 1458 0 1709 0 1.179 80 213 9966 12953 0
A#10003 -0.140941193 90 71 0 92 0 1.767 52 231 77 88 0 100 287 0 331 0 1.485 80 244 525 485 0 100 1741 0 2185 0 1.56 80 258 3330 3239 -50
A#10004 0.013363225 110 72 0 95 0 1.11 80 250 645 857 0 110 299 0 351 0 1.101 80 272 4230 4374 0 110 1798 0 2299 0 1.119 80 256 29016 31533 0
A#10005 0.271072133 110 74 0 100 0 1.353 80 291 114 181 0 110 306 0 391 0 1.299 80 339 775 909 0 110 1828 0 2417 0 1.281 80 273 5332 6806 0
A#10006 -0.086552742 100 73 0 100 0 1.17 80 253 660 818 0 100 304 0 377 0 1.143 80 253 4401 4209 0 100 1812 0 2512 0 1.149 80 247 29981 30891 0
A#10007 -0.000993175 110 72 0 97 0 1.136 80 262 429 558 0 110 293 0 364 0 1.154 80 266 2702 2832 0 110 1774 0 2456 0 1.145 80 275 18509 21216 0
A#10008 0.305006931 110 72 0 96 0 1.384 80 269 102 174 0 110 282 0 351 0 1.317 80 245 756 924 0 110 1723 0 2356 0 1.226 80 273 5304 6696 0
A#10009 100 72 0 94 0 3.181 80 262 9 9 -50 100 268 0 333 0 2.947 80 217 99 71 -50 100 1685 0 2285 0 2.625 80 233 385 400 -50
A#1001 0.240877805 100 44 0 51 0 1.725 80 315 43 68 0 100 97 0 126 0 1.858 80 316 292 346 0 100 441 0 740 0 1.685 80 309 1695 2530 0
A#10010 -0.32661673 110 71 0 93 0 1.126 80 307 428 466 0 120 268 0 333 0 1.188 120 262 2836 2398 0 120 1638 0 2260 0 1.181 120 265 19639 17206 0
A#10011 -0.44564513 110 70 0 91 0 1.225 80 291 222 216 0 110 265 0 317 0 1.24 80 251 1457 1098 0 110 1582 0 2229 0 1.246 80 260 10280 8086 0
A#10012 0.145593745 90 67 0 90 0 1.426 52 208 71 100 0 90 263 0 309 0 1.428 52 220 460 547 0 90 1517 0 2185 0 1.422 52 233 3317 3666 0
A#10013 100 67 0 87 0 3.684 80 278 -5 10 -50 100 258 0 306 0 4.011 80 274 -30 26 -50 100 1579 0 2055 0 4.68 80 267 -190 254 -50
A#10014 0.170984859 100 68 0 84 0 1.549 80 260 66 97 0 100 245 0 294 0 1.41 80 271 443 482 0 100 1521 0 1976 0 1.501 80 253 2873 3600 0

Total number of rows: 43200

Table truncated, full table size 5706 Kbytes.




Supplementary data files not provided

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